Lawrence J Parkhurst, PhD

Professor

  • 2085 Citations
  • 25 h-Index
1963 …2019

Research output per year

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Research Output

  • 2085 Citations
  • 25 h-Index
  • 80 Article
  • 2 Letter
  • 2 Review article
2019

Detailed characterization of the solution kinetics and thermodynamics of biotin, biocytin and HABA binding to avidin and streptavidin

Delgadillo, R. F., Mueser, T. C., Zaleta-Rivera, K., Carnes, K. A., González-Valdez, J. & Parkhurst, L. J., Feb 2019, In : PloS one. 14, 2, e0204194.

Research output: Contribution to journalArticle

Open Access
6 Scopus citations
2013

A small molecule directly inhibits the p53 transactivation domain from binding to replication protein A

Glanzer, J. G., Carnes, K. A., Soto, P., Liu, S., Parkhurst, L. J. & Oakley, G. G., Feb 1 2013, In : Nucleic acids research. 41, 3, p. 2047-2059 13 p.

Research output: Contribution to journalArticle

17 Scopus citations
2010

Spectroscopic properties of fluorescein and rhodamine dyes attached to DNA

Delgadillo, R. F. & Parkhurst, L. J., Mar 1 2010, In : Photochemistry and Photobiology. 86, 2, p. 261-272 12 p.

Research output: Contribution to journalArticle

17 Scopus citations
2009

The TATA-binding protein core domain in solution variably bends TATA sequences via a three-step binding mechanism

Delgadillo, R. F., Whittington, J. E., Parkhurst, L. K. & Parkhurst, L. J., Mar 3 2009, In : Biochemistry. 48, 8, p. 1801-1809 9 p.

Research output: Contribution to journalArticle

32 Scopus citations
2008

TATA-binding protein recognition and bending of a consensus promoter are protein species dependent

Whittington, J. E., Delgadillo, R. F., Attebury, T. J., Parkhurst, L. K., Daugherty, M. A. & Parkhurst, L. J., Jul 8 2008, In : Biochemistry. 47, 27, p. 7264-7273 10 p.

Research output: Contribution to journalArticle

18 Scopus citations
2006

Changes in DNA bending and flexing due to tethered cations detected by fluorescence resonance energy transfer

Williams, S. L., Parkhurst, L. K. & Parkhurst, L. J., Mar 20 2006, In : Nucleic acids research. 34, 3, p. 1028-1035 8 p.

Research output: Contribution to journalArticle

20 Scopus citations
2004
16 Scopus citations

Efficient Integration of Kinetic Differential Equation Sets Using Matrix Exponentiation

Matheson, I. B. C., Parkhurst, L. J. & DeSa, R. J., Jan 1 2004, In : Methods in enzymology. 384, p. 18-39 22 p.

Research output: Contribution to journalArticle

6 Scopus citations
2003

Native human TATA-binding protein simultaneously binds and bends promoter DNA without a slow isomerization step or TFIIB requirement

Masters, K. M., Parkhurst, K. M., Daugherty, M. A. & Parkhurst, L. J., Aug 22 2003, In : Journal of Biological Chemistry. 278, 34, p. 31685-31690 6 p.

Research output: Contribution to journalArticle

29 Scopus citations

Reflections on apparent DNA bending by charge variants of bZIP proteins

Hardwidge, P. R., Parkhurst, K. M., Parkhurst, L. J. & Maher, L. J., May 1 2003, In : Biopolymers. 69, 1, p. 110-117 8 p.

Research output: Contribution to journalReview article

9 Scopus citations
2002

Comparison of TATA-binding protein recognition of a variant and consensus DNA promoters

Powell, R. M., Parkhurst, K. M. & Parkhurst, L. J., Mar 8 2002, In : Journal of Biological Chemistry. 277, 10, p. 7776-7784 9 p.

Research output: Contribution to journalArticle

33 Scopus citations

DNA bending by bZIP charge variants: A unified study using electrophoretic phasing and fluorescence resonance energy transfer

Hardwidge, P. R., Wu, J., Williams, S. L., Parkhurst, K. M., Parkhurst, L. J. & Maher, L. J., Jun 18 2002, In : Biochemistry. 41, 24, p. 7732-7742 11 p.

Research output: Contribution to journalArticle

21 Scopus citations
2001

DNA Bends in TATA-binding Protein·TATA Complexes in Solution Are DNA Sequence-dependent

Wu, J., Parkhurst, K. M., Powell, R. M., Brenowitz, M. & Parkhurst, L. J., May 4 2001, In : Journal of Biological Chemistry. 276, 18, p. 14614-14622 9 p.

Research output: Contribution to journalArticle

66 Scopus citations

DNA Sequence-dependent Differences in TATA-binding Protein-induced DNA Bending in Solution Are Highly Sensitive to Osmolytes

Wu, J., Parkhurst, K. M., Powell, R. M. & Parkhurst, L. J., May 4 2001, In : Journal of Biological Chemistry. 276, 18, p. 14623-14627 5 p.

Research output: Contribution to journalArticle

30 Scopus citations

Marked Stepwise Differences within a Common Kinetic Mechanism Characterize TATA-binding Protein Interactions with Two Consensus Promoters

Powell, R. M., Parkhurst, K. M., Brenowitz, M. & Parkhurst, L. J., Aug 10 2001, In : Journal of Biological Chemistry. 276, 32, p. 29782-29791 10 p.

Research output: Contribution to journalArticle

30 Scopus citations

Time-resolved fluorescence resonance energy transfer studies of DNA bending in double-stranded oligonucleotides and in DNA-protein complexes

Parkhurst, L. J., Parkhurst, K. M., Powell, R., Wu, J. & Williams, S., Jan 1 2001, In : Biopolymers. 61, 3, p. 180-200 21 p.

Research output: Contribution to journalReview article

48 Scopus citations
2000

Near-ultraviolet magnetic circular dichroism spectroscopy of protein conformational states: Correlation of tryptophan band position and intensity with hemoglobin allostery

Vitale, D. J., Goldbeck, R. A., Kim-Shapiro, D. B., Esquerra, R. M., Parkhurst, L. J. & Kliger, D. S., Jun 20 2000, In : Biochemistry. 39, 24, p. 7145-7152 8 p.

Research output: Contribution to journalArticle

12 Scopus citations
1999

Intermediate species possessing bent DNA are present along the pathway to formation of a final TBP-TATA complex

Parkhurst, K. M., Richards, R. M., Brenowitz, M. & Parkhurst, L. J., Jun 25 1999, In : Journal of Molecular Biology. 289, 5, p. 1327-1341 15 p.

Research output: Contribution to journalArticle

70 Scopus citations
1996

Detection of point mutations in DNA by fluorescence energy transfer

Parkhurst, K. M. & Parkhurst, L. J., Jan 1 1996, In : Journal of Biomedical Optics. 1, 4, p. 435-441 7 p.

Research output: Contribution to journalArticle

Open Access
12 Scopus citations

Simultaneous binding and bending of promoter DNA by the TATA binding protein: Real time kinetic measurements

Parkhurst, K. M., Brenowitz, M. & Parkhurst, L. J., Jun 11 1996, In : Biochemistry. 35, 23, p. 7459-7465 7 p.

Research output: Contribution to journalArticle

116 Scopus citations
1995

A nanosecond ORD study of hemoglobin.

Parkhurst, L. J., Feb 1995, In : Biophysical journal. 68, 2, p. 399-400 2 p.

Research output: Contribution to journalArticle

Open Access
1 Scopus citations

Donor—Acceptor Distance Distributions in a Double-Labeled Fluorescent Oligonucleotide Both as a Single Strand and in Duplexes

Parkhurst, K. M. & Parkhurst, L. J., Jan 1 1995, In : Biochemistry. 34, 1, p. 293-300 8 p.

Research output: Contribution to journalArticle

73 Scopus citations
93 Scopus citations
1994

Effects of wavelength on fitting Adair constants for binding of oxygen to human hemoglobin

Parkhurst, L. J., Larsen, T. M. & Lee, H. Y., Jan 1 1994, In : Methods in enzymology. 232, C, p. 606-632 27 p.

Research output: Contribution to journalArticle

4 Scopus citations

Synthesis and Characterization of Conjugates Formed between Periodate-Oxidized Ribonucleotides and Amine-Containing Fluorophores

Hileman, R. E., Parkhurst, K. M., Gupta, N. K. & Parkhurst, L. J., Sep 1 1994, In : Bioconjugate Chemistry. 5, 5, p. 436-444 9 p.

Research output: Contribution to journalArticle

11 Scopus citations

Thermodynamic Characterization of the Cooperativity of 40S Complex Formation during the Initiation of Eukaryotic Protein Synthesis

Parkhurst, K. M., Hileman, R. E., Saha, D., Gupta, N. K. & Parkhurst, L. J., Dec 1 1994, In : Biochemistry. 33, 50, p. 15168-15177 10 p.

Research output: Contribution to journalArticle

8 Scopus citations
1993
5 Scopus citations
1992
8 Scopus citations

Rapid preparation of native alpha and beta chains of human hemoglobin

Parkhurst, K. M. & Parkhurst, L. J., Jun 1992, In : International Journal of Biochemistry. 24, 6, p. 993-998 6 p.

Research output: Contribution to journalArticle

18 Scopus citations
1991

Use of dual wavelength spectrophotometry and continuous enzymatic depletion of oxygen for determination of the oxygen binding constants of hemoglobin

Larsen, T. M., Mueser, T. C. & Parkhurst, L. J., Aug 15 1991, In : Analytical Biochemistry. 197, 1, p. 231-246 16 p.

Research output: Contribution to journalArticle

18 Scopus citations
1990

A combined nuclear magnetic resonance and absorbance stopped-flow apparatus for biochemical studies

McGee, W. A. & Parkhurst, L. J., Sep 1990, In : Analytical Biochemistry. 189, 2, p. 267-273 7 p.

Research output: Contribution to journalArticle

13 Scopus citations

A multipass cuvette for laser photolysis studies and its uses in studying hemoglobin kinetics and equilibria

Martin, K. D. & Parkhurst, L. J., May 1 1990, In : Analytical Biochemistry. 186, 2, p. 288-295 8 p.

Research output: Contribution to journalArticle

6 Scopus citations

Kinetics and thermodynamics of oxygen, CO, and azide binding by the subcomponents of soybeam leghemoglobin

Martin, K. D., Saari, L., Guang-Xin, W., Wang, T., Parkhurst, L. J. & Klucas, R. V., Dec 17 1990, In : Journal of Biological Chemistry. 265, 32, p. 19588-19593 6 p.

Research output: Contribution to journalArticle

17 Scopus citations

Kinetics and Thermodynamics of Oxygen and Carbon Monoxide Binding to the T-State Hemoglobin of Urechis caupo

Martin, K. D. & Parkhurst, L. J., Jun 19 1990, In : Biochemistry. 29, 24, p. 5718-5726 9 p.

Research output: Contribution to journalArticle

9 Scopus citations
1989

A matrix series method for the integration of rate equations in a reaction network. An alternative to Runge-Kutta methods

Zamis, T. M., Parkhurst, L. J. & Gallup, G. A., 1989, In : Computers and Chemistry. 13, 3, p. 165-171 7 p.

Research output: Contribution to journalArticle

13 Scopus citations
1986

Movement of Fe with respect to the heme plane in the R-T transition of carp hemoglobin. An extended x-ray absorption fine structure study.

Chance, M. R., Parkhurst, L. J., Powers, L. S. & Chance, B., May 5 1986, In : The Journal of biological chemistry. 261, 13, p. 5689-5692 4 p.

Research output: Contribution to journalArticle

41 Scopus citations
64 Scopus citations
1984

Cooperative Interactions in the System Ribosomes-Ribosomal Protein S1-Polynucleotide Triplets

Goss, D. J., Parkhurst, L. J., Mehta, A. M. & Wahba, A. J., Dec 1984, In : Biochemistry. 23, 26, p. 6522-6529 8 p.

Research output: Contribution to journalArticle

6 Scopus citations

Dissociation of mitochondrial malate dehydrogenase

Muller, J., Gorisch, H. & Parkhurst, L. J., Jun 28 1984, In : Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular. 787, 3, p. 258-263 6 p.

Research output: Contribution to journalArticle

5 Scopus citations
11 Scopus citations

Ligand Binding Kinetic Studies on the Hybrid Hemoglobin α(Carp):β(Human): A Hemoglobin with a Restricted Allosteric Range

Goss, D. J. & Parkhurst, L. J., May 1984, In : Biochemistry. 23, 10, p. 2174-2179 6 p.

Research output: Contribution to journalArticle

8 Scopus citations
9 Scopus citations

Oxygen Equilibrium Studies on Carp-Human Hybrid Hemoglobins

Causgrove, T., Goss, D. J. & Parkhurst, L. J., May 1984, In : Biochemistry. 23, 10, p. 2168-2173 6 p.

Research output: Contribution to journalArticle

10 Scopus citations

Studies on the role of eukaryotic nucleotide exchange factor in polypeptide chain initiation

Goss, D. J., Parkhurst, L. J., Mehta, H. B., Woodley, C. L. & Wahba, A. J., Aug 23 1984, In : Journal of Biological Chemistry. 259, 12, p. 7374-7377 4 p.

Research output: Contribution to journalArticle

37 Scopus citations

Studies on the role of the eucaryotic exchange factor in polypeptide chain initiation

Goss, D. J. & Parkhurst, L. J., Jan 1 1984, In : Federation Proceedings. 43, 6, p. no. 70

Research output: Contribution to journalArticle

1983

Kinetic and Equilibrium Studies on the Role of the β-147 Histidine in the Root Effect and Cooperativity in Carp Hemoglobin

Parkhurst, L. J., Goss, D. J., Perutz, M. F., Parkhurst, L. J., Goss, D. J. & Perutz, M. F., Jan 1 1983, In : Biochemistry. 22, 23, p. 5401-5409 9 p.

Research output: Contribution to journalArticle

39 Scopus citations

The binding of ribosomal protein SI to Sl-depleted 30S and 70S ribosomes. A fluorescence anisotropy study of the effects of Mg2+

Goss, D. J., Parkhurst, L. J., Mehta, A. M. & Wahba, A. J., Aug 25 1983, In : Nucleic acids research. 11, 16, p. 5589-5602 14 p.

Research output: Contribution to journalArticle

4 Scopus citations
1982

Kinetic studies on the interaction of chain initiation factor 3 with 70 S Escherichia coli ribosomes and subunits

Goss, D. J., Parkhurst, L. J. & Wahba, A. J., Dec 1 1982, In : Journal of Biological Chemistry. 257, 17, p. 10119-10127 9 p.

Research output: Contribution to journalArticle

24 Scopus citations
12 Scopus citations