TY - JOUR
T1 - (α-Monofluoroalkyl)phosphonates
T2 - A class of isoacidic and "tunable" mimics of biological phosphates
AU - Berkowitz, David B.
AU - Bose, Mohua
N1 - Funding Information:
Financial support from the American Heart Association is gratefully acknowledged. David B. Berkowitz is an Alfred P. Sloan Research Fellow. We would like to thank all of our coworkers who have made important contributions to this phosphate mimics project, including MariJean Eggen, Debnath Bhuniya, Quanrong Shen, Darby Sloss, Craig Semerad, Gorka Peris, Nathan Asher and Travis Pfannenstiel. Richard Shoemaker and Ronald Cerny are acknowledged for valuable technical assistance with multi-dimensional NMR and high resolution MS, respectively. This research was facilitated by grants for NMR and GC/MS instrumentation from the NIH (SIG 1-S10-RR06301) and the NSF (CHE-93000831), respectively.
PY - 2001
Y1 - 2001
N2 - In the early 1980s, Blackburn and McKenna suggested that α-fluorination might lead to phosphonates that better mimic natural phosphates. Although α-monofluorination produces phosphonates with "matching" second pKa values, the α,α-difluorinated phosphonates have received more attention in the past decade or so. Recently, reported enzyme kinetic data on the α-monofluorinated phosphonates from the O'Hagan lab and from our lab suggest that the CHF stereochemistry does affect enzyme-binding, thereby providing an additional variable that may be tuned to achieve optimal binding to an active site of interest. This asymmetry also appears in structural data from the groups of Barford/Burke and Tracey on PTP1B complexes with bound α,α-difluorinated phosphonate inhibitors. In those complexes, only one of two prochiral fluorine atoms appears to interact appreciably with the enzyme. Namely, it is thought that the pro-R (Fsi) fluorine is engaged in an important hydrogen bond with the Phe-182 amide NH. Available methods for the synthesis of this class of α-monofluorinated phosphonates are reviewed. A new convergent approach, developed at Nebraska, in which the potassium anion of (α-fluoro-α-phenylsulfonylmethyl)phosphonate is used to displace primary triflates is also described. This method is particularly convenient as it allows one to perform a "fluorinated phosphonate scan" of an active site of interest (in what follows, we use this expression to designate the synthesis and evaluation of a complete set of the CH2-, CF2- and both stereoisomeric CHF-phosphonates in an active site of interest) from a single primary triflate. The properties of the title compounds in enzyme active sites are discussed, as are possible interactions of these fluorine-containing bioisosteres with active site residues.
AB - In the early 1980s, Blackburn and McKenna suggested that α-fluorination might lead to phosphonates that better mimic natural phosphates. Although α-monofluorination produces phosphonates with "matching" second pKa values, the α,α-difluorinated phosphonates have received more attention in the past decade or so. Recently, reported enzyme kinetic data on the α-monofluorinated phosphonates from the O'Hagan lab and from our lab suggest that the CHF stereochemistry does affect enzyme-binding, thereby providing an additional variable that may be tuned to achieve optimal binding to an active site of interest. This asymmetry also appears in structural data from the groups of Barford/Burke and Tracey on PTP1B complexes with bound α,α-difluorinated phosphonate inhibitors. In those complexes, only one of two prochiral fluorine atoms appears to interact appreciably with the enzyme. Namely, it is thought that the pro-R (Fsi) fluorine is engaged in an important hydrogen bond with the Phe-182 amide NH. Available methods for the synthesis of this class of α-monofluorinated phosphonates are reviewed. A new convergent approach, developed at Nebraska, in which the potassium anion of (α-fluoro-α-phenylsulfonylmethyl)phosphonate is used to displace primary triflates is also described. This method is particularly convenient as it allows one to perform a "fluorinated phosphonate scan" of an active site of interest (in what follows, we use this expression to designate the synthesis and evaluation of a complete set of the CH2-, CF2- and both stereoisomeric CHF-phosphonates in an active site of interest) from a single primary triflate. The properties of the title compounds in enzyme active sites are discussed, as are possible interactions of these fluorine-containing bioisosteres with active site residues.
KW - (α-Monofluoroalkyl)phosphonate
KW - Glucose 6-phosphate dehydrogenase
KW - Glycerol 3-phosphate dehydrogenase
KW - Phosphate mimics
KW - Protein phosphotyrosine phosphatase PTP1B
KW - Triflate displacement
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U2 - 10.1016/S0022-1139(01)00478-X
DO - 10.1016/S0022-1139(01)00478-X
M3 - Article
AN - SCOPUS:0035169222
SN - 0022-1139
VL - 112
SP - 13
EP - 33
JO - Journal of Fluorine Chemistry
JF - Journal of Fluorine Chemistry
IS - 1
ER -