lsoelectric focusing of crude extracts demonstrated that human colonic carcinomas contained a higher proportion of β-hexosaminidase B than β-hexosaminidase A, while normal human colonic mucosa contained a higher proportion of the A form of the enzyme. Studies of the isolated isozymes showed that β-hexosaminidase B was more stable to heat and more active at low pH than β-hexosaminidase A. Kinetic studies revealed that the A and B forms of β-hexosaminidase had essentially the same Kmand Vmaxfor ρ-nitro-phenyl-N-acetyl-β-D-glucosaminide.
|Original language||English (US)|
|Number of pages||5|
|State||Published - Mar 1977|
ASJC Scopus subject areas
- Cancer Research