β-Hexosaminidase lsozymes in Human Colonic Carcinoma

Michael G. Brattain, Pamela M. Kimball, Thomas G. Pretlow

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

lsoelectric focusing of crude extracts demonstrated that human colonic carcinomas contained a higher proportion of β-hexosaminidase B than β-hexosaminidase A, while normal human colonic mucosa contained a higher proportion of the A form of the enzyme. Studies of the isolated isozymes showed that β-hexosaminidase B was more stable to heat and more active at low pH than β-hexosaminidase A. Kinetic studies revealed that the A and B forms of β-hexosaminidase had essentially the same Kmand Vmaxfor ρ-nitro-phenyl-N-acetyl-β-D-glucosaminide.

Original languageEnglish (US)
Pages (from-to)731-735
Number of pages5
JournalCancer Research
Volume37
Issue number3
StatePublished - Mar 1977

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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