1α,25-dihydroxyvitamin D3-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression

Jiang Jiang; Yang Zhang; Arup K. Indra; Gitali Ganguli-Indra; Mai N. Le; Hongjun Wang; Ronald R. Hollins; Debra A. Reilly; Mark A. Carlson; Richard L. Gallo; Adrian F. Gombart; Jingwei Xie

doi:10.2217/nnm-2018-0011

1α,25-dihydroxyvitamin D₃-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression

Jiang Jiang, Yang Zhang, Arup K. Indra, Gitali Ganguli-Indra, Mai N. Le, Hongjun Wang, Ronald R. Hollins, Debra A. Reilly, Mark A. Carlson, Richard L. Gallo, Adrian F. Gombart, Jingwei Xie

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Aim: The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃) and augmenting human CAMP induction. Materials & methods: Nanofibrous wound dressings containing 1,25(OH)₂D₃ were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo. Results: 1,25(OH)₂D₃ was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH)₂D₃ showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH)₂D₃-loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants. Conclusion: 1,25(OH)₂D₃ containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production.

Original language	English (US)
Pages (from-to)	1417-1432
Number of pages	16
Journal	Nanomedicine
Volume	13
Issue number	12
DOIs	https://doi.org/10.2217/nnm-2018-0011
State	Published - Jun 2018

Keywords

1α25-dihydroxyvitamin D
antimicrobial peptides LL-37
electrospun nanofibers
endogenous production
innate immunity
sustained delivery

ASJC Scopus subject areas

Bioengineering
Development
Materials Science(all)
Biomedical Engineering
Medicine (miscellaneous)

Access to Document

10.2217/nnm-2018-0011

Cite this

@article{e692fc79432c4e73abe98a97ef44f8f0,

title = "1α,25-dihydroxyvitamin D3-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression",

abstract = "Aim: The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) and augmenting human CAMP induction. Materials & methods: Nanofibrous wound dressings containing 1,25(OH)2D3 were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo. Results: 1,25(OH)2D3 was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH)2D3 showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH)2D3-loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants. Conclusion: 1,25(OH)2D3 containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production.",

keywords = "1α25-dihydroxyvitamin D, antimicrobial peptides LL-37, electrospun nanofibers, endogenous production, innate immunity, sustained delivery",

author = "Jiang Jiang and Yang Zhang and Indra, {Arup K.} and Gitali Ganguli-Indra and Le, {Mai N.} and Hongjun Wang and Hollins, {Ronald R.} and Reilly, {Debra A.} and Carlson, {Mark A.} and Gallo, {Richard L.} and Gombart, {Adrian F.} and Jingwei Xie",

note = "Funding Information: This work was supported by grants from the National Institute of General Medical Science (NIGMS) at the NIH (2P20 GM103480-06 and 1R01GM123081 to J Xie), the Otis Glebe Medical Research Foundation and startup funds from the University of Nebraska Medical Center. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The authors have no other relevant affiliations orfinancial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. Publisher Copyright: {\textcopyright} 2018 2018 Future Medicine Ltd.",

year = "2018",

month = jun,

doi = "10.2217/nnm-2018-0011",

language = "English (US)",

volume = "13",

pages = "1417--1432",

journal = "Nanomedicine",

issn = "1743-5889",

publisher = "Future Medicine Ltd.",

number = "12",

}

TY - JOUR

T1 - 1α,25-dihydroxyvitamin D3-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression

AU - Jiang, Jiang

AU - Zhang, Yang

AU - Indra, Arup K.

AU - Ganguli-Indra, Gitali

AU - Le, Mai N.

AU - Wang, Hongjun

AU - Hollins, Ronald R.

AU - Reilly, Debra A.

AU - Carlson, Mark A.

AU - Gallo, Richard L.

AU - Gombart, Adrian F.

AU - Xie, Jingwei

N1 - Funding Information: This work was supported by grants from the National Institute of General Medical Science (NIGMS) at the NIH (2P20 GM103480-06 and 1R01GM123081 to J Xie), the Otis Glebe Medical Research Foundation and startup funds from the University of Nebraska Medical Center. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The authors have no other relevant affiliations orfinancial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. Publisher Copyright: © 2018 2018 Future Medicine Ltd.

PY - 2018/6

Y1 - 2018/6

N2 - Aim: The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) and augmenting human CAMP induction. Materials & methods: Nanofibrous wound dressings containing 1,25(OH)2D3 were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo. Results: 1,25(OH)2D3 was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH)2D3 showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH)2D3-loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants. Conclusion: 1,25(OH)2D3 containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production.

AB - Aim: The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) and augmenting human CAMP induction. Materials & methods: Nanofibrous wound dressings containing 1,25(OH)2D3 were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo. Results: 1,25(OH)2D3 was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH)2D3 showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH)2D3-loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants. Conclusion: 1,25(OH)2D3 containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production.

KW - 1α25-dihydroxyvitamin D

KW - antimicrobial peptides LL-37

KW - electrospun nanofibers

KW - endogenous production

KW - innate immunity

KW - sustained delivery

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U2 - 10.2217/nnm-2018-0011

DO - 10.2217/nnm-2018-0011

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C2 - 29972648

AN - SCOPUS:85049566605

SN - 1743-5889

VL - 13

SP - 1417

EP - 1432

JO - Nanomedicine

JF - Nanomedicine

IS - 12

ER -