1α,25-dihydroxyvitamin D3-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression

Jiang Jiang; Yang Zhang; Arup K. Indra; Gitali Ganguli-Indra; Mai N. Le; Hongjun Wang; Ronald R. Hollins; Debra A. Reilly; Mark A. Carlson; Richard L. Gallo; Adrian F. Gombart; Jingwei Xie

doi:10.2217/nnm-2018-0011

1α,25-dihydroxyvitamin D₃-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression

Jiang Jiang, Yang Zhang, Arup K. Indra, Gitali Ganguli-Indra, Mai N. Le, Hongjun Wang, Ronald R. Hollins, Debra A. Reilly, Mark A. Carlson, Richard L. Gallo, Adrian F. Gombart, Jingwei Xie

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Aim: The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃) and augmenting human CAMP induction. Materials & methods: Nanofibrous wound dressings containing 1,25(OH)₂D₃ were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo. Results: 1,25(OH)₂D₃ was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH)₂D₃ showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH)₂D₃-loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants. Conclusion: 1,25(OH)₂D₃ containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production.

Original language	English (US)
Pages (from-to)	1417-1432
Number of pages	16
Journal	Nanomedicine
Volume	13
Issue number	12
DOIs	https://doi.org/10.2217/nnm-2018-0011
State	Published - Jun 2018

Keywords

1α25-dihydroxyvitamin D
antimicrobial peptides LL-37
electrospun nanofibers
endogenous production
innate immunity
sustained delivery

ASJC Scopus subject areas

Bioengineering
Medicine (miscellaneous)
Biomedical Engineering
General Materials Science
Development

Access to Document

10.2217/nnm-2018-0011

Cite this

@article{e692fc79432c4e73abe98a97ef44f8f0,

title = "1α,25-dihydroxyvitamin D3-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression",

abstract = "Aim: The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) and augmenting human CAMP induction. Materials & methods: Nanofibrous wound dressings containing 1,25(OH)2D3 were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo. Results: 1,25(OH)2D3 was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH)2D3 showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH)2D3-loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants. Conclusion: 1,25(OH)2D3 containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production.",

keywords = "1α25-dihydroxyvitamin D, antimicrobial peptides LL-37, electrospun nanofibers, endogenous production, innate immunity, sustained delivery",

author = "Jiang Jiang and Yang Zhang and Indra, {Arup K.} and Gitali Ganguli-Indra and Le, {Mai N.} and Hongjun Wang and Hollins, {Ronald R.} and Reilly, {Debra A.} and Carlson, {Mark A.} and Gallo, {Richard L.} and Gombart, {Adrian F.} and Jingwei Xie",

note = "Publisher Copyright: {\textcopyright} 2018 2018 Future Medicine Ltd.",

year = "2018",

month = jun,

doi = "10.2217/nnm-2018-0011",

language = "English (US)",

volume = "13",

pages = "1417--1432",

journal = "Nanomedicine",

issn = "1743-5889",

publisher = "Taylor and Francis Ltd.",

number = "12",

}

TY - JOUR

T1 - 1α,25-dihydroxyvitamin D3-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression

AU - Jiang, Jiang

AU - Zhang, Yang

AU - Indra, Arup K.

AU - Ganguli-Indra, Gitali

AU - Le, Mai N.

AU - Wang, Hongjun

AU - Hollins, Ronald R.

AU - Reilly, Debra A.

AU - Carlson, Mark A.

AU - Gallo, Richard L.

AU - Gombart, Adrian F.

AU - Xie, Jingwei

PY - 2018/6

Y1 - 2018/6

N2 - Aim: The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) and augmenting human CAMP induction. Materials & methods: Nanofibrous wound dressings containing 1,25(OH)2D3 were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo. Results: 1,25(OH)2D3 was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH)2D3 showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH)2D3-loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants. Conclusion: 1,25(OH)2D3 containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production.

AB - Aim: The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) and augmenting human CAMP induction. Materials & methods: Nanofibrous wound dressings containing 1,25(OH)2D3 were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo. Results: 1,25(OH)2D3 was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH)2D3 showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH)2D3-loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants. Conclusion: 1,25(OH)2D3 containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production.

KW - 1α25-dihydroxyvitamin D

KW - antimicrobial peptides LL-37

KW - electrospun nanofibers

KW - endogenous production

KW - innate immunity

KW - sustained delivery

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