1α,25-dihydroxyvitamin D3-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression

Jiang Jiang, Yang Zhang, Arup K. Indra, Gitali Ganguli-Indra, Mai N. Le, Hongjun Wang, Ronald R. Hollins, Debra A. Reilly, Mark A. Carlson, Richard L. Gallo, Adrian F. Gombart, Jingwei Xie

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Aim: The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) and augmenting human CAMP induction. Materials & methods: Nanofibrous wound dressings containing 1,25(OH)2D3 were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo. Results: 1,25(OH)2D3 was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH)2D3 showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH)2D3-loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants. Conclusion: 1,25(OH)2D3 containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production.

Original languageEnglish (US)
Pages (from-to)1417-1432
Number of pages16
JournalNanomedicine
Volume13
Issue number12
DOIs
StatePublished - Jun 2018

Keywords

  • 1α25-dihydroxyvitamin D
  • antimicrobial peptides LL-37
  • electrospun nanofibers
  • endogenous production
  • innate immunity
  • sustained delivery

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)

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