Purified rat liver nuclei contain enzymes capable of activating polycyclic aromatic hydrocarbons which can then bind to nuclear DNA. This has been demonstrated by incubating nuclei with [14C] hydrocarbon and purifying the nuclear DNA containing bound hydrocarbon. Intraperitoneal injection of the rats with 3-methylcholanthrene twenty-four hours before livers were removed raised the level of hydrocarbon binding to nuclear DNA four-fold. Omission of reduced nicotinamide adenine dinucleotide phosphate from incubation mixtures caused a four-fold decrease in the ability of 3-methylcholanthrene induced nuclei to bind hydrocarbon to nuclear DNA. The most potent carcinogens in a series of seven hydrocarbons displayed a relatively higher extent of binding to nuclear DNA. The data suggest that nuclear aryl hydroxylases effect covalent binding of hydrocarbons to DNA and presumably initiate the cancer process.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jun 18 1974|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology