TY - JOUR
T1 - 3D porous chitosan-alginate scaffolds promote proliferation and enrichment of cancer stem-like cells
AU - Florczyk, Stephen J.
AU - Kievit, Forrest M.
AU - Wang, Kui
AU - Erickson, Ariane E.
AU - Ellenbogen, Richard G.
AU - Zhang, Miqin
N1 - Funding Information:
This work was supported in part by NIH grants R01CA172455 and a Kyocera Professorship Endowment. K. W. acknowledges support from the University of Washington College of Engineering Dean's Fellowship. We acknowledge the use of the SEM at the Dept. of Materials Science and Engineering, the confocal and optical microscopes at Keck Microscopy Imaging Facility, and the flow cytometers at the Dept. of Immunology at the University of Washington. We acknowledge laboratory assistance from Ms Allison M. Lewis in preparing CA scaffolds. We thank Benjamin S. Glick for submitting the RFP plasmid to AddGene.
Publisher Copyright:
© 2016 The Royal Society of Chemistry.
PY - 2016
Y1 - 2016
N2 - Cancer stem cells are increasingly becoming a primary target for new cancer treatment development. The ability to study their transient behavior in vitro will provide the opportunity for high-throughput testing of more effective therapies. We have previously demonstrated the use of 3D porous chitosan-alginate (CA) scaffolds to promote cancer stem-like cell (CSC) proliferation and enrichment in glioblastoma. Here we use 3D porous CA scaffolds to promote cancer stem-like cell enrichment in cell lines from prostate, liver, and breast cancers, and investigate the proliferation, morphology, and gene expressions of cells cultured in CA scaffolds as compared to 2D controls. The 3D CA scaffold cultures for all three cancer types showed reduced proliferation, formation of tumor spheroids, and increased expression of CSC associated mark genes (CD133 and NANOG), as opposed to monolayers. Additionally, we present a putative mechanism for the cancer stem-like cell enrichment on CA scaffolds. This study demonstrates that the cancer stem-like cell enrichment in CA scaffolds is a robust process that is not restricted to particular cancer types.
AB - Cancer stem cells are increasingly becoming a primary target for new cancer treatment development. The ability to study their transient behavior in vitro will provide the opportunity for high-throughput testing of more effective therapies. We have previously demonstrated the use of 3D porous chitosan-alginate (CA) scaffolds to promote cancer stem-like cell (CSC) proliferation and enrichment in glioblastoma. Here we use 3D porous CA scaffolds to promote cancer stem-like cell enrichment in cell lines from prostate, liver, and breast cancers, and investigate the proliferation, morphology, and gene expressions of cells cultured in CA scaffolds as compared to 2D controls. The 3D CA scaffold cultures for all three cancer types showed reduced proliferation, formation of tumor spheroids, and increased expression of CSC associated mark genes (CD133 and NANOG), as opposed to monolayers. Additionally, we present a putative mechanism for the cancer stem-like cell enrichment on CA scaffolds. This study demonstrates that the cancer stem-like cell enrichment in CA scaffolds is a robust process that is not restricted to particular cancer types.
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U2 - 10.1039/c6tb01713d
DO - 10.1039/c6tb01713d
M3 - Article
AN - SCOPUS:84989294391
SN - 2050-7518
VL - 4
SP - 6326
EP - 6334
JO - Journal of Materials Chemistry B
JF - Journal of Materials Chemistry B
IS - 38
ER -