3D printing of multilayered scaffolds for rotator cuff tendon regeneration

Xiping Jiang, Shaohua Wu, Mitchell Kuss, Yunfan Kong, Wen Shi, Philipp N. Streubel, Tieshi Li, Bin Duan

Research output: Contribution to journalArticlepeer-review

65 Scopus citations


Repairing massive rotator cuff tendon defects remains a challenge due to the high retear rate after surgical intervention. 3D printing has emerged as a promising technique that enables the fabrication of engineered tissues with heterogeneous structures and mechanical properties, as well as controllable microenvironments for tendon regeneration. In this study, we developed a new strategy for rotator cuff tendon repair by combining a 3D printed scaffold of polylactic-co-glycolic acid (PLGA) with cell-laden collagen-fibrin hydrogels. We designed and fabricated two types of scaffolds: one featuring a separate layer-by-layer structure and another with a tri-layered structure as a whole. Uniaxial tensile tests showed that both types of scaffolds had improved mechanical properties compared to single-layered PLGA scaffolds. The printed scaffold with collagen-fibrin hydrogels effectively supported the growth, proliferation, and tenogenic differentiation of human adipose-derived mesenchymal stem cells. Subcutaneous implantation of the multilayered scaffolds demonstrated their excellent in vivo biocompatibility. This study demonstrates the feasibility of 3D printing multilayered scaffolds for application in rotator cuff tendon regeneration.

Original languageEnglish (US)
Pages (from-to)636-643
Number of pages8
JournalBioactive Materials
Issue number3
StatePublished - Sep 2020


  • Collagen-fibrin hydrogel
  • Tendon repair
  • Tenogenic differentiation
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biomedical Engineering


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