@article{5afab3dd1a444e24ad764e1cf1c9f71f,
title = "3D printing of silk fibroin-based hybrid scaffold treated with platelet rich plasma for bone tissue engineering",
abstract = "3D printing/bioprinting are promising techniques to fabricate scaffolds with well controlled and patient-specific structures and architectures for bone tissue engineering. In this study, we developed a composite bioink consisting of silk fibroin (SF), gelatin (GEL), hyaluronic acid (HA), and tricalcium phosphate (TCP) and 3D bioprinted the silk fibroin-based hybrid scaffolds. The 3D bioprinted scaffolds with dual crosslinking were further treated with human platelet-rich plasma (PRP) to generate PRP coated scaffolds. Live/Dead and MTT assays demonstrated that PRP treatment could obviously promote the cell growth and proliferation of human adipose derived mesenchymal stem cells (HADMSC). In addition, the treatment of PRP did not significantly affect alkaline phosphatase (ALP) activity and expression, but significantly upregulated the gene expression levels of late osteogenic markers. This study demonstrated that the 3D printing of silk fibroin-based hybrid scaffolds, in combination with PRP post-treatment, might be a more efficient strategy to promote osteogenic differentiation of adult stem cells and has significant potential to be used for bone tissue engineering.",
keywords = "3D bioprinting, Coating, Growth factor cocktail, Hybrid scaffold, Tissue engineering",
author = "Liang Wei and Shaohua Wu and Mitchell Kuss and Xiping Jiang and Runjun Sun and Patrick Reid and Xiaohong Qin and Bin Duan",
note = "Funding Information: This work is supported by National Institutes of Health ( R01 AR073225 ) to Dr. Bin Duan; ( R21AI140026 ) to Drs Patrick Reid and Bin Duan; Chinese Universities Scientific Fund ( CUSF-DH-D-2016008 ), China Scholarship Council , Doctoral Program of Xi'an Polytechnic University ( BS201902 ) to Dr. Liang Wei. The authors would like to thank Tom Bargar and Nicholas Conoan of the Electron Microscopy Core Facility (EMCF) at the University of Nebraska Medical Center for technical assistance. The EMCF is supported by state funds from the Nebraska Research Initiative (NRI) and the University of Nebraska Foundation , and institutionally by the Office of the Vice Chancellor for Research. Funding Information: This work is supported by National Institutes of Health (R01 AR073225) to Dr. Bin Duan; (R21AI140026) to Drs Patrick Reid and Bin Duan; Chinese Universities Scientific Fund (CUSF-DH-D-2016008), China Scholarship Council, Doctoral Program of Xi'an Polytechnic University (BS201902) to Dr. Liang Wei. The authors would like to thank Tom Bargar and Nicholas Conoan of the Electron Microscopy Core Facility (EMCF) at the University of Nebraska Medical Center for technical assistance. The EMCF is supported by state funds from the Nebraska Research Initiative (NRI) and the University of Nebraska Foundation, and institutionally by the Office of the Vice Chancellor for Research. Publisher Copyright: {\textcopyright} 2019",
year = "2019",
month = dec,
doi = "10.1016/j.bioactmat.2019.09.001",
language = "English (US)",
volume = "4",
pages = "256--260",
journal = "Bioactive Materials",
issn = "2452-199X",
publisher = "KeAi Communications Co",
}