5-Azacytidine-resistant mutants of Chlorella virus IL-3A

Dwight E. Burbank; Shelly L. Shields; Anne M. Schuster; James L. Van Etten

doi:10.1016/0042-6822(90)90261-O

5-Azacytidine-resistant mutants of Chlorella virus IL-3A

Dwight E. Burbank, Shelly L. Shields, Anne M. Schuster, James L. Van Etten

Plant Pathology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Many dsDNA-containing viruses which infect the unicellular, eukaryotic Chlorella-like green alga strain NC64A encode for DNA methyltransferases and DNA site-specific (restriction) endonucleases. We have hypothesized that these endonucleases help degrade host DNA permitting deoxynucleotides to recycle into virus DNA. This hypothesis was tested by isolating deletion mutants of Chlorella virus IL-3A lacking functional genes for the cytosine methyltransferase M.CviJI and the cognate site-specific endonuclease CviJI. The growth and burst sizes of the mutants and parent virus were identical. Also host nuclear and chloroplast DNAs disappeared from infected cells at the same rates. Thus M.CviJI and CviJI activities are not required for IL-3A replication and CviJI activity is not essential for host DNA degradation.

Original language	English (US)
Pages (from-to)	311-315
Number of pages	5
Journal	Virology
Volume	176
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(90)90261-O
State	Published - May 1990

ASJC Scopus subject areas

Virology

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10.1016/0042-6822(90)90261-O

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@article{4e093b62cc8a4a93898c4d228c46374f,

title = "5-Azacytidine-resistant mutants of Chlorella virus IL-3A",

abstract = "Many dsDNA-containing viruses which infect the unicellular, eukaryotic Chlorella-like green alga strain NC64A encode for DNA methyltransferases and DNA site-specific (restriction) endonucleases. We have hypothesized that these endonucleases help degrade host DNA permitting deoxynucleotides to recycle into virus DNA. This hypothesis was tested by isolating deletion mutants of Chlorella virus IL-3A lacking functional genes for the cytosine methyltransferase M.CviJI and the cognate site-specific endonuclease CviJI. The growth and burst sizes of the mutants and parent virus were identical. Also host nuclear and chloroplast DNAs disappeared from infected cells at the same rates. Thus M.CviJI and CviJI activities are not required for IL-3A replication and CviJI activity is not essential for host DNA degradation.",

author = "Burbank, {Dwight E.} and Shields, {Shelly L.} and Schuster, {Anne M.} and {Van Etten}, {James L.}",

note = "Funding Information: We thank Susan Spilker for technical assistance, Stan Hattman and David Swinton for the base analyses, Lawrence Bogarad for plasmid pZmclO0. and Les Lane and Roy French for helpful discussions. This investigation was supported, in part, by Public Health Service Grant GM-32441 from the National Institute of General Medicine and Grant DE-AC02-82ER12086 from the Department of Energy.",

year = "1990",

month = may,

doi = "10.1016/0042-6822(90)90261-O",

language = "English (US)",

volume = "176",

pages = "311--315",

journal = "Virology",

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publisher = "Academic Press Inc.",

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T1 - 5-Azacytidine-resistant mutants of Chlorella virus IL-3A

AU - Burbank, Dwight E.

AU - Shields, Shelly L.

AU - Schuster, Anne M.

AU - Van Etten, James L.

N1 - Funding Information: We thank Susan Spilker for technical assistance, Stan Hattman and David Swinton for the base analyses, Lawrence Bogarad for plasmid pZmclO0. and Les Lane and Roy French for helpful discussions. This investigation was supported, in part, by Public Health Service Grant GM-32441 from the National Institute of General Medicine and Grant DE-AC02-82ER12086 from the Department of Energy.

PY - 1990/5

Y1 - 1990/5

N2 - Many dsDNA-containing viruses which infect the unicellular, eukaryotic Chlorella-like green alga strain NC64A encode for DNA methyltransferases and DNA site-specific (restriction) endonucleases. We have hypothesized that these endonucleases help degrade host DNA permitting deoxynucleotides to recycle into virus DNA. This hypothesis was tested by isolating deletion mutants of Chlorella virus IL-3A lacking functional genes for the cytosine methyltransferase M.CviJI and the cognate site-specific endonuclease CviJI. The growth and burst sizes of the mutants and parent virus were identical. Also host nuclear and chloroplast DNAs disappeared from infected cells at the same rates. Thus M.CviJI and CviJI activities are not required for IL-3A replication and CviJI activity is not essential for host DNA degradation.

AB - Many dsDNA-containing viruses which infect the unicellular, eukaryotic Chlorella-like green alga strain NC64A encode for DNA methyltransferases and DNA site-specific (restriction) endonucleases. We have hypothesized that these endonucleases help degrade host DNA permitting deoxynucleotides to recycle into virus DNA. This hypothesis was tested by isolating deletion mutants of Chlorella virus IL-3A lacking functional genes for the cytosine methyltransferase M.CviJI and the cognate site-specific endonuclease CviJI. The growth and burst sizes of the mutants and parent virus were identical. Also host nuclear and chloroplast DNAs disappeared from infected cells at the same rates. Thus M.CviJI and CviJI activities are not required for IL-3A replication and CviJI activity is not essential for host DNA degradation.

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5-Azacytidine-resistant mutants of Chlorella virus IL-3A

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