A broadscale phylogenetic analysis of group ii intron rnas and intron-encoded reverse transcriptases

Dawn M. Simon, Scot A. Kelchner, Steven Zimmerly

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Group II introns are self-splicing RNAs that are frequently assumed to be the ancestors of spliceosomal introns. They are widely distributed in bacteria and are also found in organelles of plants, fungi, and protists. In this study, we present a broadscale phylogenetic analysis of group II introns using sequence data from both the conserved RNA structure and the intron-encoded reverse transcriptase (RT). Two similar phylogenies are estimated for the RT open reading frame (ORF), based on either amino acid or nucleotide sequence, whereas one phylogeny is produced for the RNA. In making these estimates, we confronted nearly all the classic challenges to phylogenetic inference, including positional saturation, base composition heterogeneity, short internodes with low support, and sensitivity to taxon sampling. Although the major lineages are well-defined, robust resolution of topology is not possible between these lineages. The approximately unbiased (AU) and Shimodaira-Hasegawa topology tests indicated that the RT ORF and RNA ribozyme data sets are in significant conflict under a variety of models, revealing the possibility of imperfect coevolution between group II introns and their intron-encoded ORFs. The high level of sequence divergence, large timescale, and limited number of alignable characters in our study are representative of many RTs and group I introns, and our results suggest that phylogenetic analyses of any of these sequences could suffer from the same sources of error and instability identified in this study.

Original languageEnglish (US)
Pages (from-to)2795-2808
Number of pages14
JournalMolecular biology and evolution
Volume26
Issue number12
DOIs
StatePublished - Dec 1 2009

Keywords

  • Base composition heterogeneity
  • Group II intron
  • Phylogeny
  • Retroelement
  • Reverse transcriptase
  • Taxon sampling

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'A broadscale phylogenetic analysis of group ii intron rnas and intron-encoded reverse transcriptases'. Together they form a unique fingerprint.

  • Cite this