A comparison of 2-amino-4-phosphonobutyric acid (AP4) receptors and [3H]AP4 binding sites in the rat brain

Richard J. Bridges, Tim J. Hearn, Daniel T. Monaghan, Carl W. Cotman

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

The glutamate analogue 2-amino-4-phosphonobutyric acid (AP4) is a potent antagonist at several synapses where an excitatory amino acid appears to be the neurotransmitter. Previous studies identified a Cl-/Ca2+ dependent [3H]glutamate binding site in synaptic plasma membrane (SPM) preparations that was also labeled by [3H]AP4 and exhibited a pharmacology similar to the AP4 receptor. This report examines the pharmacological specificity in both biochemical and electrophysiological preparations in greater detail. Several compounds are identified which readily interact with the apparent binding site in membranes, but neither mimic nor inhibit the action of AP4 in electrophysiological studies. The rate of dissociation of [3H]AP4 from SPMs is shown to increase in the presence of added AP4 and increasing the osmolarity in the SPM binding assay decreases the level of observed [3H]AP4 binding. These finding indicate both a heterogenous population of binding sites and the occurrence of transport. It is concluded that much of the AP4 binding observed in SPM preparations is to a site other than the AP4 receptor. The results provide a further pharmacological description of AP4 receptors which should facilitate the identification of the receptor in biochemical preparations.

Original languageEnglish (US)
Pages (from-to)204-209
Number of pages6
JournalBrain Research
Volume375
Issue number1
DOIs
StatePublished - Jun 4 1986

Keywords

  • 2-amino-4-phosphonobutyric acid (AP4) receptor
  • glutamate binding
  • glutamate receptor
  • glutamate uptake
  • lateral perforant path

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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