A comparison of molluscan and arthropod hemocyanin-I. Circular dichroism and absorption spectra

Kenneth W. Nickerson, Kensal E. Van Holde

Research output: Contribution to journalArticle

206 Scopus citations

Abstract

1. 1. The circular dichroic (CD) spectra of molluscan and arthropod oxyhemocyanins are qualitatively and quantitatively different. 2. 2. Molluscan oxyhemocyanins have three CD bands in the visible, a positive band near 450 nm, a negative band near 570 nm and a positive band near 710 nm. 3. 3. Arthropod oxyhemocyanins have only two visible CD bands, both positive, near 490 and 610 nm. 4. 4. Both types have a negative CD band near 340 nm. 5. 5. In the region between 260-300 nm, the CD of molluscan hemocyanins is positive, that of arthropods negative. 6. 6. These data indicate fundamental differences in the oxygen binding sites of these related proteins.

Original languageEnglish (US)
Pages (from-to)855-872
Number of pages18
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume39
Issue number4
DOIs
StatePublished - Aug 15 1971

Keywords

  • Absorption spectra
  • Bohr effect
  • N-bromosuccinimide
  • circular dichroism
  • conformational changes in proteins
  • evolution of proteins
  • extinction coefficients of proteins
  • hemocyanins
  • oxygen binding
  • proteins, concentration determination
  • tryptophan oxidation

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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