A concise review of quantification methods for determination of vitamin K in various biological matrices

Yuning Zhang, Veenu Bala, Zhihao Mao, Yashpal S. Chhonker, Daryl J. Murry

Research output: Contribution to journalReview articlepeer-review

25 Scopus citations


Vitamin K is an essential nutrient in the body and involved in numerous physiological and pathophysiological functions. Both the lack and surplus of vitamin K can put human health at risk. Therefore, it becomes necessary to monitor vitamin K concentrations in different biomatrices through establishing sensitive and specific analytical methods. This review collectively describes an updated overview of the sample pretreatment methodologies and methods for quantitative determination of vitamin K that have been used in last two decades. High Performance Liquid Chromatography (HPLC) is commonly utilized as a standard for separation of vitamin K in combination with different detection including spectroscopic, spectrometric, fluorometric and mass spectroscopy. Recent progress in sample pretreatment technologies and quantitation methodologies have enhanced the ability to identify and quantitate vitamin K in biomatrices to further advance our understanding of the role of this vitamin in human health and disease.

Original languageEnglish (US)
Pages (from-to)133-141
Number of pages9
JournalJournal of Pharmaceutical and Biomedical Analysis
StatePublished - May 30 2019


  • Biological samples
  • Chromatography
  • High performance liquid chromatography
  • Menaquinones
  • Phylloquinone
  • Vitamin K

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry


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