A diagnosis strategy to determine the Shiga toxin-producing Escherichia coli O157 status of pens of feedlot cattle

D. R. Smith, J. T. Gray, R. A. Moxley, S. M. Younts-Dahl, M. P. Blackford, S. Hinkley, L. L. Hungerford, C. T. Milton, T. J. Klopfenstein

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Although cattle are reservoirs, no validated method exists to monitor Shiga toxin-producing Escherichia coli O157 (STEC O157) on farms. In 29 Midwestern United States feedlot pens we compared culturing faeces from the individual cattle to: (1) culturing rope devices that cattle rub or chew; and (2) culturing a composite of faecal pats. Eighty-six per cent (68-96%) of pens were classified correctly using rope devices to detect pens with at least 16% of the cattle shedding STEC O157 [sensitivity=82% (57-96%); specificity=92% (62-100%)]. Ninety per cent of pens (73-98%) were classified correctly using composite faeces to detect pens with at least 37% of the cattle shedding STEC O157 [sensitivity=86% (42-100%); specificity=91% (71-99%)]. Ranking pens into three risk levels based on parallel interpretation of the pen-test results correlated (Spearman's r=0.76, P<0.0001) with the pen's prevalence. This strategy could identify pens of cattle posing a higher risk to food safety.

Original languageEnglish (US)
Pages (from-to)297-302
Number of pages6
JournalEpidemiology and Infection
Volume132
Issue number2
DOIs
StatePublished - Apr 2004

ASJC Scopus subject areas

  • Epidemiology
  • Infectious Diseases

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