A dot enzyme immunoassay for detection of IgM antibodies against phenolic glycolipid-I sera from leprosy patients

S. Kumar, K. D. Moudgil, A. H. Band, P. R. Naraynan, S. K. Gupta, A. K. Sharma, G. P. Talwar

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A visual dipstick dot enzyme immunoassay (EIA) for diagnosis of leprosy is described. The assay is based on detection of IgM antibodies against phenolic glycolipid (PGL-I) in sera from leprosy patients. The antigen (PGL-I or synthetic disaccharide of PGL-I) was dotted on a nitrocellulose pad stuck on a plastic strip (dipstick). Sera were used at a dilution of 1:200. Peroxidase coupled mouse anti-human IgM monoclonal antibodies were used as the conjugate. A positive test gave a blue dot against a white background. The test was highly specific for leprosy, and was quite sensitive for detection of bacilliferous (BL/LL) leprosy. The antigen dotted and preblocked dipsticks stored at room temperature upto 4 months of observation period, were unable in the assay.

Original languageEnglish (US)
Pages (from-to)185-190
Number of pages6
JournalIndian Journal of Leprosy
Volume58
Issue number2
StatePublished - 1986

ASJC Scopus subject areas

  • Dermatology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'A dot enzyme immunoassay for detection of IgM antibodies against phenolic glycolipid-I sera from leprosy patients'. Together they form a unique fingerprint.

Cite this