Abstract
A visual dipstick dot enzyme immunoassay (EIA) for diagnosis of leprosy is described. The assay is based on detection of IgM antibodies against phenolic glycolipid (PGL-I) in sera from leprosy patients. The antigen (PGL-I or synthetic disaccharide of PGL-I) was dotted on a nitrocellulose pad stuck on a plastic strip (dipstick). Sera were used at a dilution of 1:200. Peroxidase coupled mouse anti-human IgM monoclonal antibodies were used as the conjugate. A positive test gave a blue dot against a white background. The test was highly specific for leprosy, and was quite sensitive for detection of bacilliferous (BL/LL) leprosy. The antigen dotted and preblocked dipsticks stored at room temperature upto 4 months of observation period, were unable in the assay.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 185-190 |
| Number of pages | 6 |
| Journal | Indian Journal of Leprosy |
| Volume | 58 |
| Issue number | 2 |
| State | Published - 1986 |
| Externally published | Yes |
ASJC Scopus subject areas
- Dermatology
- Infectious Diseases