A dynamic, ring-forming bactofilin critical for maintaining cell size in the obligate intracellular bacterium Chlamydia trachomatis

Mary R. Brockett, Junghoon Lee, John V. Cox, George W. Liechti, Scot P. Ouellette

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Bactofilins are polymer-forming cytoskeletal proteins that are widely conserved in bacteria. Members of this protein family have diverse functional roles such as orienting subcellular molecular processes, establishing cell polarity, and aiding in cell shape maintenance. Using sequence alignment to the conserved bactofilin domain, we identified a bactofilin ortholog, BacACT, in the obligate intracellular pathogen Chlamydia trachomatis. Chlamydia species are obligate intracellular bacteria that undergo a developmental cycle alternating between infectious nondividing elementary bodies (EBs) and noninfectious dividing reticulate bodies (RBs). As Chlamydia divides by a polarized division process, we hypothesized that BacACT may function to establish polarity in these unique bacteria. Utilizing a combination of fusion constructs and high-resolution fluorescence microscopy, we determined that BacACT forms dynamic, membrane-associated filament- and ring-like structures in Chlamydia's replicative RB form. Contrary to our hypothesis, these structures are distinct from the microbe's cell division machinery and do not colocalize with septal peptidoglycan or MreB, the major organizer of the bacterium's division complex. Bacterial two-hybrid assays demonstrated BacACT interacts homotypically but does not directly interact with proteins involved in cell division or peptidoglycan biosynthesis. To investigate the function of BacACT in chlamydial development, we constructed a conditional knockdown strain using a newly developed CRISPR interference system. We observed that reducing bacACT expression significantly increased chlamydial cell size. Normal RB morphology was restored when an additional copy of bacACT was expressed in trans during knockdown. These data reveal a novel function for chlamydial bactofilin in maintaining cell size in this obligate intracellular bacterium.

Original languageEnglish (US)
Article numbere00203-21
JournalInfection and immunity
Volume89
Issue number8
DOIs
StatePublished - Jul 15 2021

Keywords

  • Bactofilin
  • Cell division
  • Cell morphology
  • Cell polarity
  • Chlamydia

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'A dynamic, ring-forming bactofilin critical for maintaining cell size in the obligate intracellular bacterium Chlamydia trachomatis'. Together they form a unique fingerprint.

Cite this