A facile system for encoding unnatural amino acids in mammalian cells

Peng R. Chen; Dan Groff; Jiantao Guo; Weijia Ou; Susan Cellitti; Bernhard H. Geierstanger; Peter G. Schultz

doi:10.1002/anie.200900683

A facile system for encoding unnatural amino acids in mammalian cells

Peng R. Chen, Dan Groff, Jiantao Guo, Weijia Ou, Susan Cellitti, Bernhard H. Geierstanger, Peter G. Schultz

Research output: Contribution to journal › Article › peer-review

246 Scopus citations

Abstract

A shuttle system has been developed to genetically encode unnatural amino acids in mammalian cells using aminoacyl-tRNA synthetases (aaRSs) evolved in E. coli. A pyrrolysyl-tRNA synthetase (PylRS) mutant was evolved in E. coli that selectively aminoacylates a cognate nonsense suppressor tRNA with a photocaged lysine derivative. Transfer of this orthogonal tRNA-aaRS pair into mammalian cells made possible the selective incorporation of this unnatural amino acid into proteins.

Original language	English (US)
Pages (from-to)	4052-4055
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	48
Issue number	22
DOIs	https://doi.org/10.1002/anie.200900683
State	Published - May 18 2009
Externally published	Yes

Keywords

Gene expression
Konnatural amino acids
Photocaged lysine
tRNA

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.200900683

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AU - Ou, Weijia

AU - Cellitti, Susan

AU - Geierstanger, Bernhard H.

AU - Schultz, Peter G.

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AB - A shuttle system has been developed to genetically encode unnatural amino acids in mammalian cells using aminoacyl-tRNA synthetases (aaRSs) evolved in E. coli. A pyrrolysyl-tRNA synthetase (PylRS) mutant was evolved in E. coli that selectively aminoacylates a cognate nonsense suppressor tRNA with a photocaged lysine derivative. Transfer of this orthogonal tRNA-aaRS pair into mammalian cells made possible the selective incorporation of this unnatural amino acid into proteins.

KW - Gene expression

KW - Konnatural amino acids

KW - Photocaged lysine

KW - tRNA

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A facile system for encoding unnatural amino acids in mammalian cells

Abstract

Keywords

ASJC Scopus subject areas

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