Abstract
A shuttle system has been developed to genetically encode unnatural amino acids in mammalian cells using aminoacyl-tRNA synthetases (aaRSs) evolved in E. coli. A pyrrolysyl-tRNA synthetase (PylRS) mutant was evolved in E. coli that selectively aminoacylates a cognate nonsense suppressor tRNA with a photocaged lysine derivative. Transfer of this orthogonal tRNA-aaRS pair into mammalian cells made possible the selective incorporation of this unnatural amino acid into proteins.
Original language | English (US) |
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Pages (from-to) | 4052-4055 |
Number of pages | 4 |
Journal | Angewandte Chemie - International Edition |
Volume | 48 |
Issue number | 22 |
DOIs | |
State | Published - May 18 2009 |
Externally published | Yes |
Keywords
- Gene expression
- Konnatural amino acids
- Photocaged lysine
- tRNA
ASJC Scopus subject areas
- Catalysis
- General Chemistry