TY - JOUR
T1 - A four-season longitudinal study of enterohaemorrhagic Escherichia coli in beef cow–calf herds in Mississippi and Nebraska
AU - Schneider, L. G.
AU - Lewis, G. L.
AU - Moxley, R. A.
AU - Smith, D. R.
N1 - Funding Information:
This work is supported by Agriculture and Food Research Initiative grant no. 2012-68003-30155 from the USDA National Institute of Food and Agriculture This is a contribution of the Beef Cattle Health and Reproduction Program at Mississippi State University, supported by the Mikell and Mary Cheek Hall Davis Endowment for Beef Cattle Health and Reproduction. The authors would like to thank Dr. Rhonda Vann, Dr. Jane Parish, Dr. Kris Hubbard, Christina Loftin, Katie Sanford and Cheyenne Stuart for technical assistance,
Funding Information:
This work is supported by Agriculture and Food Research Initiative grant no. 2012-68003-30155 from the USDA National Institute of Food and Agriculture This is a contribution of the Beef Cattle Health and Reproduction Program at Mississippi State University, supported by the Mikell and Mary Cheek Hall Davis Endowment for Beef Cattle Health and Reproduction. The authors would like to thank Dr. Rhonda Vann, Dr. Jane Parish, Dr. Kris Hubbard, Christina Loftin, Katie Sanford and Cheyenne Stuart for technical assistance, as well as the technicians in Dr. Rodney Moxley's laboratory at UNL, and the participating herds.
Publisher Copyright:
© 2018 Blackwell Verlag GmbH
PY - 2018/8
Y1 - 2018/8
N2 - Our objective was to describe the probability of detecting seven serogroups of enterohaemorrhagic Escherichia coli (EHEC-7) of public health importance in faecal samples from beef cow–calf herds and to test for factors associated with their detection. Fresh faecal samples (n = 85) from two Mississippi and two Nebraska herds were collected in each of four seasons. Samples were tested for each EHEC-7 serogroup by a molecular screening assay. Separate management groups within herds were sampled, and group-level factors were recorded. To measure the effects of factors on faecal shedding of EHEC-7, separate multivariable logistic regression models were used, accounting for the random effect of clustering by group within farm. Statistical significance was set α = 0.05. Fifty-nine samples (4.3%) were positive for EHEC O26, and Nebraska samples were more likely to be positive than Mississippi samples (OR = 12.4, 95% CI: 1.1, 139.2). Forty-four samples (3.2%) were positive for EHEC O45. Odds for detection were greater in the summer than all other seasons combined (OR = 4.2, 95% CI: 1.3, 14.0), and odds decreased if a precipitation event occurred (OR = 0.07, 95% CI: 0.006, 0.8). EHEC O103 was detected in 66 samples (4.9%) with increased probability to be detected at increased temperature. EHEC O111 was detected in 71 samples (5.2%), and 43 samples (3.2%) were positive for EHEC O145. Both EHEC O111 and O145 were associated separately with season, with greater probability for detection in the summer. Eighteen (1.3%) and 68 (5.0%) samples were positive for EHEC O121 and EHEC O157, respectively. We failed to detect significant explanatory factors associated with probability to detect EHEC O121 or O157. Factors that vary by time and place, such as precipitation, ambient temperature, region and season, are uniquely associated with the probability to detect EHEC-7 in fresh faeces collected from cow–calf herds.
AB - Our objective was to describe the probability of detecting seven serogroups of enterohaemorrhagic Escherichia coli (EHEC-7) of public health importance in faecal samples from beef cow–calf herds and to test for factors associated with their detection. Fresh faecal samples (n = 85) from two Mississippi and two Nebraska herds were collected in each of four seasons. Samples were tested for each EHEC-7 serogroup by a molecular screening assay. Separate management groups within herds were sampled, and group-level factors were recorded. To measure the effects of factors on faecal shedding of EHEC-7, separate multivariable logistic regression models were used, accounting for the random effect of clustering by group within farm. Statistical significance was set α = 0.05. Fifty-nine samples (4.3%) were positive for EHEC O26, and Nebraska samples were more likely to be positive than Mississippi samples (OR = 12.4, 95% CI: 1.1, 139.2). Forty-four samples (3.2%) were positive for EHEC O45. Odds for detection were greater in the summer than all other seasons combined (OR = 4.2, 95% CI: 1.3, 14.0), and odds decreased if a precipitation event occurred (OR = 0.07, 95% CI: 0.006, 0.8). EHEC O103 was detected in 66 samples (4.9%) with increased probability to be detected at increased temperature. EHEC O111 was detected in 71 samples (5.2%), and 43 samples (3.2%) were positive for EHEC O145. Both EHEC O111 and O145 were associated separately with season, with greater probability for detection in the summer. Eighteen (1.3%) and 68 (5.0%) samples were positive for EHEC O121 and EHEC O157, respectively. We failed to detect significant explanatory factors associated with probability to detect EHEC O121 or O157. Factors that vary by time and place, such as precipitation, ambient temperature, region and season, are uniquely associated with the probability to detect EHEC-7 in fresh faeces collected from cow–calf herds.
KW - Escherichia coli spp.
KW - cattle
KW - cow–calf
KW - enterohaemorrhagic Escherichia coli
KW - epidemiology
KW - foodborne pathogens
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U2 - 10.1111/zph.12465
DO - 10.1111/zph.12465
M3 - Article
C2 - 29573177
AN - SCOPUS:85044301920
SN - 1863-1959
VL - 65
SP - 552
EP - 559
JO - Zoonoses and Public Health
JF - Zoonoses and Public Health
IS - 5
ER -