@article{365287a9160b4d2791d099afccbdc8d4,
title = "A genetically encoded cyclobutene probe for labelling of live cells",
abstract = "We have identified an aminoacyl-tRNA synthetase/tRNA pair for the efficient and site-specific incorporation of a cyclobutene-containing amino acid into proteins in response to an amber nonsense codon. Fast and fluorescent labeling of purified proteins and intact proteins in live cells was demonstrated using the inverse electron demand Diels-Alder reaction with a tetrazine.",
author = "K. Liu and B. Enns and B. Evans and N. Wang and X. Shang and W. Sittiwong and Dussault, {P. H.} and J. Guo",
note = "Funding Information: This work was supported by the National Institute of Health (grant 1R01AI111862 to J. G.), National Science Foundation (grant 1553041 to J. G., and EPS-1004094 to P. D.), and the Life Sciences Interdisciplinary Research Program (Univ. of Nebraska-Lincoln). Portions of this research were performed in facilities renovated with support from the NIH (RR016544). The authors would like to acknowledge assistance from the following individuals at the University of Nebraska-Lincoln: Prof. Ron Cerny (Nebraska Center for Mass Spectrometry, protein mass spectrometry); Dr Sophie Alvarez (Proteomics and Metabolomics Facility); Prof. Martha Morton (Chemistry Research Instrumentation Facility, kinetics); Prof. David Berkowitz (Department of Chemistry, dissolving metal reduction); Dr You Zhou (Center for Biotechnology Microscopy facility, fluorescence imaging). Publisher Copyright: {\textcopyright} 2017 The Royal Society of Chemistry.",
year = "2017",
doi = "10.1039/c7cc05580c",
language = "English (US)",
volume = "53",
pages = "10604--10607",
journal = "Chemical Communications",
issn = "1359-7345",
publisher = "Royal Society of Chemistry",
number = "76",
}