A genetically encoded cyclobutene probe for labelling of live cells

K. Liu, B. Enns, B. Evans, N. Wang, X. Shang, W. Sittiwong, P. H. Dussault, J. Guo

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


We have identified an aminoacyl-tRNA synthetase/tRNA pair for the efficient and site-specific incorporation of a cyclobutene-containing amino acid into proteins in response to an amber nonsense codon. Fast and fluorescent labeling of purified proteins and intact proteins in live cells was demonstrated using the inverse electron demand Diels-Alder reaction with a tetrazine.

Original languageEnglish (US)
Pages (from-to)10604-10607
Number of pages4
JournalChemical Communications
Issue number76
StatePublished - 2017

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry


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