A genetically encoded fluorescent probe in mammalian cells

Abhishek Chatterjee, Jiantao Guo, Hyun Soo Lee, Peter G. Schultz

Research output: Contribution to journalArticlepeer-review

97 Scopus citations

Abstract

Fluorescent reporters are useful in vitro and in vivo probes of protein structure, function, and localization. Here we report that the fluorescent amino acid, 3-(6-acetylnaphthalen-2-ylamino)-2-aminopropanoic acid (Anap), can be site-specifically incorporated into proteins in mammalian cells in response to the TAG codon with high efficiency using an orthogonal amber suppressor tRNA/aminoacyl-tRNA synthetase (aaRS) pair. We further demonstrate that Anap can be used to image the subcellular localization of proteins in live mammalian cells. The small size of Anap, its environment-sensitive fluorescence, and the ability to introduce Anap at specific sites in the proteome by simple mutagenesis make it a unique and valuable tool in eukaryotic cell biology.

Original languageEnglish (US)
Pages (from-to)12540-12543
Number of pages4
JournalJournal of the American Chemical Society
Volume135
Issue number34
DOIs
StatePublished - Aug 28 2013

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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