A group M consensus envelope glycoprotein induces antibodies that neutralize subsets of subtype B and C HIV-1 primary viruses

Hua Xin Liao; Laura L. Sutherland; Shi Mao Xia; Mary E. Brock; Richard M. Scearce; Stacie Vanleeuwen; S. Munir Alam; Mildred McAdams; Eric A. Weaver; Zenaido T. Camacho; Ben Jiang Ma; Yingying Li; Julie M. Decker; Gary J. Nabel; David C. Montefiori; Beatrice H. Hahn; Bette T. Korber; Feng Gao; Barton F. Haynes

doi:10.1016/j.virol.2006.04.043

A group M consensus envelope glycoprotein induces antibodies that neutralize subsets of subtype B and C HIV-1 primary viruses

Hua Xin Liao, Laura L. Sutherland, Shi Mao Xia, Mary E. Brock, Richard M. Scearce, Stacie Vanleeuwen, S. Munir Alam, Mildred McAdams, Eric A. Weaver, Zenaido T. Camacho, Ben Jiang Ma, Yingying Li, Julie M. Decker, Gary J. Nabel, David C. Montefiori, Beatrice H. Hahn, Bette T. Korber, Feng Gao, Barton F. Haynes

Research output: Contribution to journal › Article › peer-review

168 Scopus citations

Abstract

HIV-1 subtype C is the most common HIV-1 group M subtype in Africa and many parts of Asia. However, to date HIV-1 vaccine candidate immunogens have not induced potent and broadly neutralizing antibodies against subtype C primary isolates. We have used a centralized gene strategy to address HIV-1 diversity and generated a group M consensus envelope gene with shortened consensus variable loops (CON-S) for comparative studies with wild-type (WT) Env immunogens. Our results indicate that the consensus HIV-1 group M CON-S Env elicited cross-subtype neutralizing antibodies of similar or greater breadth and titer than the WT Envs tested, indicating the utility of a centralized gene strategy. Our study also shows the feasibility of iterative improvements in Env immunogenicity by rational design of centralized genes.

Original language	English (US)
Pages (from-to)	268-282
Number of pages	15
Journal	Virology
Volume	353
Issue number	2
DOIs	https://doi.org/10.1016/j.virol.2006.04.043
State	Published - Sep 30 2006
Externally published	Yes

Keywords

Consensus immunogens
HIV-1
Neutralization antibody
Vaccine

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2006.04.043

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Liao, H. X., Sutherland, L. L., Xia, S. M., Brock, M. E., Scearce, R. M., Vanleeuwen, S., Alam, S. M., McAdams, M., Weaver, E. A., Camacho, Z. T., Ma, B. J., Li, Y., Decker, J. M., Nabel, G. J., Montefiori, D. C., Hahn, B. H., Korber, B. T., Gao, F., & Haynes, B. F. (2006). A group M consensus envelope glycoprotein induces antibodies that neutralize subsets of subtype B and C HIV-1 primary viruses. Virology, 353(2), 268-282. https://doi.org/10.1016/j.virol.2006.04.043

Liao, HX, Sutherland, LL, Xia, SM, Brock, ME, Scearce, RM, Vanleeuwen, S, Alam, SM, McAdams, M, Weaver, EA, Camacho, ZT, Ma, BJ, Li, Y, Decker, JM, Nabel, GJ, Montefiori, DC, Hahn, BH, Korber, BT, Gao, F & Haynes, BF 2006, 'A group M consensus envelope glycoprotein induces antibodies that neutralize subsets of subtype B and C HIV-1 primary viruses', Virology, vol. 353, no. 2, pp. 268-282. https://doi.org/10.1016/j.virol.2006.04.043

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title = "A group M consensus envelope glycoprotein induces antibodies that neutralize subsets of subtype B and C HIV-1 primary viruses",

abstract = "HIV-1 subtype C is the most common HIV-1 group M subtype in Africa and many parts of Asia. However, to date HIV-1 vaccine candidate immunogens have not induced potent and broadly neutralizing antibodies against subtype C primary isolates. We have used a centralized gene strategy to address HIV-1 diversity and generated a group M consensus envelope gene with shortened consensus variable loops (CON-S) for comparative studies with wild-type (WT) Env immunogens. Our results indicate that the consensus HIV-1 group M CON-S Env elicited cross-subtype neutralizing antibodies of similar or greater breadth and titer than the WT Envs tested, indicating the utility of a centralized gene strategy. Our study also shows the feasibility of iterative improvements in Env immunogenicity by rational design of centralized genes.",

keywords = "Consensus immunogens, HIV-1, Neutralization antibody, Vaccine",

author = "Liao, {Hua Xin} and Sutherland, {Laura L.} and Xia, {Shi Mao} and Brock, {Mary E.} and Scearce, {Richard M.} and Stacie Vanleeuwen and Alam, {S. Munir} and Mildred McAdams and Weaver, {Eric A.} and Camacho, {Zenaido T.} and Ma, {Ben Jiang} and Yingying Li and Decker, {Julie M.} and Nabel, {Gary J.} and Montefiori, {David C.} and Hahn, {Beatrice H.} and Korber, {Bette T.} and Feng Gao and Haynes, {Barton F.}",

note = "Funding Information: The authors thank Robert Parks and Christopher Ryan for expert technical assistance, James Robinson, Hermann Katinger, Dennis Burton, and the NIAID AIDS Reagent Repository for reagents and Kim McClammy for expert secretarial assistance. Supported by NIH grants PO1 AI52816, P30 AI51445, HIVRAD PO-1, AI061734, NO1 AI 85338, R21AI55386, and PO1 AI 28147. ",

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AU - Liao, Hua Xin

AU - Sutherland, Laura L.

AU - Xia, Shi Mao

AU - Brock, Mary E.

AU - Scearce, Richard M.

AU - Vanleeuwen, Stacie

AU - Alam, S. Munir

AU - McAdams, Mildred

AU - Weaver, Eric A.

AU - Camacho, Zenaido T.

AU - Ma, Ben Jiang

AU - Li, Yingying

AU - Decker, Julie M.

AU - Nabel, Gary J.

AU - Montefiori, David C.

AU - Hahn, Beatrice H.

AU - Korber, Bette T.

AU - Gao, Feng

AU - Haynes, Barton F.

N1 - Funding Information: The authors thank Robert Parks and Christopher Ryan for expert technical assistance, James Robinson, Hermann Katinger, Dennis Burton, and the NIAID AIDS Reagent Repository for reagents and Kim McClammy for expert secretarial assistance. Supported by NIH grants PO1 AI52816, P30 AI51445, HIVRAD PO-1, AI061734, NO1 AI 85338, R21AI55386, and PO1 AI 28147.

PY - 2006/9/30

Y1 - 2006/9/30

N2 - HIV-1 subtype C is the most common HIV-1 group M subtype in Africa and many parts of Asia. However, to date HIV-1 vaccine candidate immunogens have not induced potent and broadly neutralizing antibodies against subtype C primary isolates. We have used a centralized gene strategy to address HIV-1 diversity and generated a group M consensus envelope gene with shortened consensus variable loops (CON-S) for comparative studies with wild-type (WT) Env immunogens. Our results indicate that the consensus HIV-1 group M CON-S Env elicited cross-subtype neutralizing antibodies of similar or greater breadth and titer than the WT Envs tested, indicating the utility of a centralized gene strategy. Our study also shows the feasibility of iterative improvements in Env immunogenicity by rational design of centralized genes.

AB - HIV-1 subtype C is the most common HIV-1 group M subtype in Africa and many parts of Asia. However, to date HIV-1 vaccine candidate immunogens have not induced potent and broadly neutralizing antibodies against subtype C primary isolates. We have used a centralized gene strategy to address HIV-1 diversity and generated a group M consensus envelope gene with shortened consensus variable loops (CON-S) for comparative studies with wild-type (WT) Env immunogens. Our results indicate that the consensus HIV-1 group M CON-S Env elicited cross-subtype neutralizing antibodies of similar or greater breadth and titer than the WT Envs tested, indicating the utility of a centralized gene strategy. Our study also shows the feasibility of iterative improvements in Env immunogenicity by rational design of centralized genes.

KW - Consensus immunogens

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KW - Neutralization antibody

KW - Vaccine

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A group M consensus envelope glycoprotein induces antibodies that neutralize subsets of subtype B and C HIV-1 primary viruses

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Keywords

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