A Hg(II) INDUCED CONFORMATIONAL CHANGE IN PENICILLINASE

Leo D'Souza; Madaiah Madaiah; Richard A. Day; Kenneth W. Nickerson

doi:10.1111/j.1399-3011.1975.tb02440.x

A Hg(II) INDUCED CONFORMATIONAL CHANGE IN PENICILLINASE

Leo D'Souza, Madaiah Madaiah, Richard A. Day, Kenneth W. Nickerson

Biological Sciences

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Penicillinase (E.C.3.5.2.6) from Bacillus cereus 569/H is inhibited by Hg(II). The inhibition is characterized by non‐competitive kinetics and can be reversed by EDTA. A Hg(II) induced conformational change is indicated because: (1) The EDTA regenerated activity is unstable and is rapidly converted to an iodine‐sensitive state, and (2) An irreversible change in the circular dichroism spectrum at 222 nm is found.

Original language	English (US)
Pages (from-to)	251-259
Number of pages	9
Journal	International Journal of Peptide and Protein Research
Volume	7
Issue number	3
DOIs	https://doi.org/10.1111/j.1399-3011.1975.tb02440.x
State	Published - May 1975

ASJC Scopus subject areas

Biochemistry

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10.1111/j.1399-3011.1975.tb02440.x

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abstract = "Penicillinase (E.C.3.5.2.6) from Bacillus cereus 569/H is inhibited by Hg(II). The inhibition is characterized by non‐competitive kinetics and can be reversed by EDTA. A Hg(II) induced conformational change is indicated because: (1) The EDTA regenerated activity is unstable and is rapidly converted to an iodine‐sensitive state, and (2) An irreversible change in the circular dichroism spectrum at 222 nm is found.",

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AU - Day, Richard A.

AU - Nickerson, Kenneth W.

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N2 - Penicillinase (E.C.3.5.2.6) from Bacillus cereus 569/H is inhibited by Hg(II). The inhibition is characterized by non‐competitive kinetics and can be reversed by EDTA. A Hg(II) induced conformational change is indicated because: (1) The EDTA regenerated activity is unstable and is rapidly converted to an iodine‐sensitive state, and (2) An irreversible change in the circular dichroism spectrum at 222 nm is found.

AB - Penicillinase (E.C.3.5.2.6) from Bacillus cereus 569/H is inhibited by Hg(II). The inhibition is characterized by non‐competitive kinetics and can be reversed by EDTA. A Hg(II) induced conformational change is indicated because: (1) The EDTA regenerated activity is unstable and is rapidly converted to an iodine‐sensitive state, and (2) An irreversible change in the circular dichroism spectrum at 222 nm is found.

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A Hg(II) INDUCED CONFORMATIONAL CHANGE IN PENICILLINASE

Abstract

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