A highly conserved family of inactivated archaeal B family DNA polymerases

Igor B. Rogozin; Kira S. Makarova; Youri I. Pavlov; Eugene V. Koonin

doi:10.1186/1745-6150-3-32

A highly conserved family of inactivated archaeal B family DNA polymerases

Igor B. Rogozin, Kira S. Makarova, Youri I. Pavlov, Eugene V. Koonin

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36 Scopus citations

Abstract

A widespread and highly conserved family of apparently inactivated derivatives of archaeal B-family DNA polymerases is described. Phylogenetic analysis shows that the inactivated forms comprise a distinct clade among archaeal B-family polymerases and that, within this clade, Euryarchaea and Crenarchaea are clearly separated from each other and from a small group of bacterial homologs. These findings are compatible with an ancient duplication of the DNA polymerase gene followed by inactivation and parallel loss in some of the lineages although contribution of horizontal gene transfer cannot be ruled out. The inactivated derivative of the archaeal DNA polymerase could form a complex with the active paralog and play a structural role in DNA replication.

Original language	English (US)
Article number	32
Journal	Biology direct
Volume	3
DOIs	https://doi.org/10.1186/1745-6150-3-32
State	Published - Aug 6 2008

ASJC Scopus subject areas

Immunology
Ecology, Evolution, Behavior and Systematics
Modeling and Simulation
General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences
Applied Mathematics

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title = "A highly conserved family of inactivated archaeal B family DNA polymerases",

abstract = "A widespread and highly conserved family of apparently inactivated derivatives of archaeal B-family DNA polymerases is described. Phylogenetic analysis shows that the inactivated forms comprise a distinct clade among archaeal B-family polymerases and that, within this clade, Euryarchaea and Crenarchaea are clearly separated from each other and from a small group of bacterial homologs. These findings are compatible with an ancient duplication of the DNA polymerase gene followed by inactivation and parallel loss in some of the lineages although contribution of horizontal gene transfer cannot be ruled out. The inactivated derivative of the archaeal DNA polymerase could form a complex with the active paralog and play a structural role in DNA replication.",

author = "Rogozin, {Igor B.} and Makarova, {Kira S.} and Pavlov, {Youri I.} and Koonin, {Eugene V.}",

note = "Funding Information: The research of IBR, KSM and EVK is supported by the Department of Health and Human Services intramural program (NIH, National Library of Medicine). YIP is supported by the NIH grant 1R01CA129925-01A1, Nebraska DHHS grant LB506 and Eppley Institute Pilot Project grant.",

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doi = "10.1186/1745-6150-3-32",

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T1 - A highly conserved family of inactivated archaeal B family DNA polymerases

AU - Rogozin, Igor B.

AU - Makarova, Kira S.

AU - Pavlov, Youri I.

AU - Koonin, Eugene V.

N1 - Funding Information: The research of IBR, KSM and EVK is supported by the Department of Health and Human Services intramural program (NIH, National Library of Medicine). YIP is supported by the NIH grant 1R01CA129925-01A1, Nebraska DHHS grant LB506 and Eppley Institute Pilot Project grant.

PY - 2008/8/6

Y1 - 2008/8/6

N2 - A widespread and highly conserved family of apparently inactivated derivatives of archaeal B-family DNA polymerases is described. Phylogenetic analysis shows that the inactivated forms comprise a distinct clade among archaeal B-family polymerases and that, within this clade, Euryarchaea and Crenarchaea are clearly separated from each other and from a small group of bacterial homologs. These findings are compatible with an ancient duplication of the DNA polymerase gene followed by inactivation and parallel loss in some of the lineages although contribution of horizontal gene transfer cannot be ruled out. The inactivated derivative of the archaeal DNA polymerase could form a complex with the active paralog and play a structural role in DNA replication.

AB - A widespread and highly conserved family of apparently inactivated derivatives of archaeal B-family DNA polymerases is described. Phylogenetic analysis shows that the inactivated forms comprise a distinct clade among archaeal B-family polymerases and that, within this clade, Euryarchaea and Crenarchaea are clearly separated from each other and from a small group of bacterial homologs. These findings are compatible with an ancient duplication of the DNA polymerase gene followed by inactivation and parallel loss in some of the lineages although contribution of horizontal gene transfer cannot be ruled out. The inactivated derivative of the archaeal DNA polymerase could form a complex with the active paralog and play a structural role in DNA replication.

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A highly conserved family of inactivated archaeal B family DNA polymerases

Abstract

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