@article{9a828040167e4473a32819782ed9e3d8,
title = "A Highly Efficacious Electrical Biofilm Treatment System for Combating Chronic Wound Bacterial Infections",
abstract = "Biofilm infection has a high prevalence in chronic wounds and can delay wound healing. Current treatment using debridement and antibiotic administration imposes a significant burden on patients and healthcare systems. To address their limitations, a highly efficacious electrical antibiofilm treatment system is described in this paper. This system uses high-intensity current (75 mA cm−2) to completely debride biofilm above the wound surface and enhance antibiotic delivery into biofilm-infected wounds simultaneously. Combining these two effects, this system uses short treatments (≤2 h) to reduce bacterial count of methicillin-resistant S. aureus (MRSA) biofilm-infected ex vivo skin wounds from 1010 to 105.2 colony-forming units (CFU) g−1. Taking advantage of the hydrogel ionic circuit design, this system enhances the in vivo safety of high-intensity current application compared to conventional devices. The in vivo antibiofilm efficacy of the system is tested using a diabetic mouse-based wound infection model. MRSA biofilm bacterial count decreases from 109.0 to 104.6 CFU g−1 at 1 day post-treatment and to 103.3 CFU g−1 at 7 days post-treatment, both of which are below the clinical threshold for infection. Overall, this novel technology provides a quick, safe, yet highly efficacious treatment to chronic wound biofilm infections.",
keywords = "biofilm, chronic wound infection, electrical debridement, hydrogel ionic circuit, iontophoretic antibiotic delivery",
author = "Fan Zhao and Yajuan Su and Junying Wang and Svetlana Romanova and DiMaio, {Dominick J.} and Jingwei Xie and Siwei Zhao",
note = "Funding Information: This research was supported by Departmental Start‐Up Fund provided by the Holland Regenerative Medicine Program at the University of Nebraska Medical Center. This research was also supported by the COBRE Nebraska Center for Nanomedicine pilot grant (funded by NIGMS P30GM127200); NIAMS R21 AR078439; NIAMS R21 AR080906; and NIGMS R01 GM138552. The content in this publication was solely the responsibility of the authors and did not necessarily represent the official views of the NIH. The authors acknowledge Dr. Wen Xue for her assistance in in vitro cell viability test; Dr. Donghee Lee for his assistance in fluorescent microscopy; Dr. Bing Xue for paraffin section and histological staining of skin tissues; Dr. Wen Shi for his assistance in high‐performance liquid chromatography (HPLC); and Dr. Noel Johnson for his assistance in in vivo safety study. Funding Information: This research was supported by Departmental Start-Up Fund provided by the Holland Regenerative Medicine Program at the University of Nebraska Medical Center. This research was also supported by the COBRE Nebraska Center for Nanomedicine pilot grant (funded by NIGMS P30GM127200); NIAMS R21 AR078439; NIAMS R21 AR080906; and NIGMS R01 GM138552. The content in this publication was solely the responsibility of the authors and did not necessarily represent the official views of the NIH. The authors acknowledge Dr. Wen Xue for her assistance in in vitro cell viability test; Dr. Donghee Lee for his assistance in fluorescent microscopy; Dr. Bing Xue for paraffin section and histological staining of skin tissues; Dr. Wen Shi for his assistance in high-performance liquid chromatography (HPLC); and Dr. Noel Johnson for his assistance in in vivo safety study. Publisher Copyright: {\textcopyright} 2022 Wiley-VCH GmbH.",
year = "2023",
month = feb,
day = "9",
doi = "10.1002/adma.202208069",
language = "English (US)",
volume = "35",
journal = "Advanced Materials",
issn = "0935-9648",
publisher = "Wiley-VCH Verlag",
number = "6",
}