The steady state kinetics of pig liver glucose dehydrogenase with NAD, NADP, d-glucose, and d-xylose as substrates is reported. Alternate substrate, product inhibition, and dead-end inhibition studies support an ordered Bi Bi mechanism as is the case with arabinose (fucose) dehydrogenase from the same source. Only NADH acts as an inhibitor, whereas NADPH, xylolactone, and gluconolactone exhibit no product inhibition. Insulin and glucagon have no effect on glucose dehydrogenase kinetics in vitro.
ASJC Scopus subject areas
- Molecular Biology