TY - JOUR
T1 - A kinetic study of pig liver glucose dehydrogenase
AU - Carper, W. Robert
AU - Toews, Myron L.
AU - Thompson, Richard E.
AU - Buess, Charles M.
PY - 1976/7
Y1 - 1976/7
N2 - The steady state kinetics of pig liver glucose dehydrogenase with NAD, NADP, d-glucose, and d-xylose as substrates is reported. Alternate substrate, product inhibition, and dead-end inhibition studies support an ordered Bi Bi mechanism as is the case with arabinose (fucose) dehydrogenase from the same source. Only NADH acts as an inhibitor, whereas NADPH, xylolactone, and gluconolactone exhibit no product inhibition. Insulin and glucagon have no effect on glucose dehydrogenase kinetics in vitro.
AB - The steady state kinetics of pig liver glucose dehydrogenase with NAD, NADP, d-glucose, and d-xylose as substrates is reported. Alternate substrate, product inhibition, and dead-end inhibition studies support an ordered Bi Bi mechanism as is the case with arabinose (fucose) dehydrogenase from the same source. Only NADH acts as an inhibitor, whereas NADPH, xylolactone, and gluconolactone exhibit no product inhibition. Insulin and glucagon have no effect on glucose dehydrogenase kinetics in vitro.
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U2 - 10.1016/0003-9861(76)90513-0
DO - 10.1016/0003-9861(76)90513-0
M3 - Article
C2 - 8012
AN - SCOPUS:0017081186
SN - 0003-9861
VL - 175
SP - 312
EP - 320
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 1
ER -