A leucine-rich repeat protein of carrot that exhibits antifreeze activity

Knut Meyer; Michael Keil; Michael J. Naldrett

doi:10.1016/S0014-5793(99)00280-X

A leucine-rich repeat protein of carrot that exhibits antifreeze activity

Knut Meyer, Michael Keil, Michael J. Naldrett

Research output: Contribution to journal › Article › peer-review

109 Scopus citations

Abstract

A gene encoding an antifreeze protein (AFP) was isolated from carrot (Daucus carota) using sequence information derived from the purified protein. The carrot AFP is highly similar to the polygalacturonase inhibitor protein (PGIP) family of apoplastic plant leucine-rich repeat (LRR) proteins. Expression of the AFP gene is rapidly induced by low temperatures. Furthermore, expression of the AFP gene in transgenic Arabidopsis thaliana plants leads to an accumulation of antifreeze activity. Our findings suggest that a new type of plant antifreeze protein has recently evolved from PGIPs. Copyright (C) 1999 Federation of European Biochemical Societies.

Original language	English (US)
Pages (from-to)	171-178
Number of pages	8
Journal	FEBS Letters
Volume	447
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-5793(99)00280-X
State	Published - Mar 26 1999
Externally published	Yes

Keywords

Antifreeze protein
Apoplast
Arabidopsis thaliana
Daucus carota
Leucine-rich repeat protein
Polygalacturonase inhibitor protein

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(99)00280-X

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title = "A leucine-rich repeat protein of carrot that exhibits antifreeze activity",

abstract = "A gene encoding an antifreeze protein (AFP) was isolated from carrot (Daucus carota) using sequence information derived from the purified protein. The carrot AFP is highly similar to the polygalacturonase inhibitor protein (PGIP) family of apoplastic plant leucine-rich repeat (LRR) proteins. Expression of the AFP gene is rapidly induced by low temperatures. Furthermore, expression of the AFP gene in transgenic Arabidopsis thaliana plants leads to an accumulation of antifreeze activity. Our findings suggest that a new type of plant antifreeze protein has recently evolved from PGIPs. Copyright (C) 1999 Federation of European Biochemical Societies.",

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T1 - A leucine-rich repeat protein of carrot that exhibits antifreeze activity

AU - Meyer, Knut

AU - Keil, Michael

AU - Naldrett, Michael J.

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N2 - A gene encoding an antifreeze protein (AFP) was isolated from carrot (Daucus carota) using sequence information derived from the purified protein. The carrot AFP is highly similar to the polygalacturonase inhibitor protein (PGIP) family of apoplastic plant leucine-rich repeat (LRR) proteins. Expression of the AFP gene is rapidly induced by low temperatures. Furthermore, expression of the AFP gene in transgenic Arabidopsis thaliana plants leads to an accumulation of antifreeze activity. Our findings suggest that a new type of plant antifreeze protein has recently evolved from PGIPs. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - A gene encoding an antifreeze protein (AFP) was isolated from carrot (Daucus carota) using sequence information derived from the purified protein. The carrot AFP is highly similar to the polygalacturonase inhibitor protein (PGIP) family of apoplastic plant leucine-rich repeat (LRR) proteins. Expression of the AFP gene is rapidly induced by low temperatures. Furthermore, expression of the AFP gene in transgenic Arabidopsis thaliana plants leads to an accumulation of antifreeze activity. Our findings suggest that a new type of plant antifreeze protein has recently evolved from PGIPs. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - Antifreeze protein

KW - Apoplast

KW - Arabidopsis thaliana

KW - Daucus carota

KW - Leucine-rich repeat protein

KW - Polygalacturonase inhibitor protein

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A leucine-rich repeat protein of carrot that exhibits antifreeze activity

Abstract

Keywords

ASJC Scopus subject areas

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