A live attenuated Listeria monocytogenes vaccine vector expressing SIV Gag is safe and immunogenic in macaques and can be administered repeatedly

Gaia Sciaranghella, Samir K. Lakhashe, Mila Ayash-Rashkovsky, Saied Mirshahidi, Nagadenahalli B. Siddappa, Francis J. Novembre, Vijayakumar Velu, Rama Rao Amara, Chenghui Zhou, Sufen Li, Zhongxia Li, Fred R. Frankel, Ruth M. Ruprecht

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Listeria monocytogenes (Lm) is known to induce strong cellular immune responses. We constructed a live-attenuated Lm vector, Lmdd-BdopSIVgag, which encodes SIVmac239 gag. Intragastric (i.g.) administration of 3×1012 bacteria to rhesus macaques was safe and induced anti-Gag cellular but no humoral immune responses. Boosting of Gag-specific cellular responses was observed after i.g. administration of Lmdd-BdopSIVgag to previously vaccinated RM despite preexisting anti-Lm immunity shown by lymphoproliferative responses. Surprisingly, anti-Lm cellular responses were also detected in non-vaccinated controls, which may reflect the fact that Lm is a ubiquitous bacterium. The novel, live-attenuated Lmdd-BdopSIVgag may be an attractive platform for oral vaccine delivery.

Original languageEnglish (US)
Pages (from-to)476-486
Number of pages11
JournalVaccine
Volume29
Issue number3
DOIs
StatePublished - Jan 10 2011

Keywords

  • Anti-vector immunity
  • Cellular immune responses
  • HIV/AIDS vaccine
  • Live attenuated Listeria monocytogenes
  • Live oral vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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  • Cite this

    Sciaranghella, G., Lakhashe, S. K., Ayash-Rashkovsky, M., Mirshahidi, S., Siddappa, N. B., Novembre, F. J., Velu, V., Amara, R. R., Zhou, C., Li, S., Li, Z., Frankel, F. R., & Ruprecht, R. M. (2011). A live attenuated Listeria monocytogenes vaccine vector expressing SIV Gag is safe and immunogenic in macaques and can be administered repeatedly. Vaccine, 29(3), 476-486. https://doi.org/10.1016/j.vaccine.2010.10.072