TY - JOUR
T1 - A live attenuated Listeria monocytogenes vaccine vector expressing SIV Gag is safe and immunogenic in macaques and can be administered repeatedly
AU - Sciaranghella, Gaia
AU - Lakhashe, Samir K.
AU - Ayash-Rashkovsky, Mila
AU - Mirshahidi, Saied
AU - Siddappa, Nagadenahalli B.
AU - Novembre, Francis J.
AU - Velu, Vijayakumar
AU - Amara, Rama Rao
AU - Zhou, Chenghui
AU - Li, Sufen
AU - Li, Zhongxia
AU - Frankel, Fred R.
AU - Ruprecht, Ruth M.
N1 - Funding Information:
We thank Elizabeth Samit for assistance with the preparation of this manuscript, Stephanie Ehnert for the coordination of the primate studies, and Dr. Richard Raybourne (formerly of the Food and Drug Administration) for assistance and protocols to assess anti-Lm immune responses. This study was supported by NIH grant P01 AI054558 to FF and RMR.
PY - 2011/1/10
Y1 - 2011/1/10
N2 - Listeria monocytogenes (Lm) is known to induce strong cellular immune responses. We constructed a live-attenuated Lm vector, Lmdd-BdopSIVgag, which encodes SIVmac239 gag. Intragastric (i.g.) administration of 3×1012 bacteria to rhesus macaques was safe and induced anti-Gag cellular but no humoral immune responses. Boosting of Gag-specific cellular responses was observed after i.g. administration of Lmdd-BdopSIVgag to previously vaccinated RM despite preexisting anti-Lm immunity shown by lymphoproliferative responses. Surprisingly, anti-Lm cellular responses were also detected in non-vaccinated controls, which may reflect the fact that Lm is a ubiquitous bacterium. The novel, live-attenuated Lmdd-BdopSIVgag may be an attractive platform for oral vaccine delivery.
AB - Listeria monocytogenes (Lm) is known to induce strong cellular immune responses. We constructed a live-attenuated Lm vector, Lmdd-BdopSIVgag, which encodes SIVmac239 gag. Intragastric (i.g.) administration of 3×1012 bacteria to rhesus macaques was safe and induced anti-Gag cellular but no humoral immune responses. Boosting of Gag-specific cellular responses was observed after i.g. administration of Lmdd-BdopSIVgag to previously vaccinated RM despite preexisting anti-Lm immunity shown by lymphoproliferative responses. Surprisingly, anti-Lm cellular responses were also detected in non-vaccinated controls, which may reflect the fact that Lm is a ubiquitous bacterium. The novel, live-attenuated Lmdd-BdopSIVgag may be an attractive platform for oral vaccine delivery.
KW - Anti-vector immunity
KW - Cellular immune responses
KW - HIV/AIDS vaccine
KW - Live attenuated Listeria monocytogenes
KW - Live oral vaccine
UR - http://www.scopus.com/inward/record.url?scp=78650518250&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78650518250&partnerID=8YFLogxK
U2 - 10.1016/j.vaccine.2010.10.072
DO - 10.1016/j.vaccine.2010.10.072
M3 - Article
C2 - 21070847
AN - SCOPUS:78650518250
SN - 0264-410X
VL - 29
SP - 476
EP - 486
JO - Vaccine
JF - Vaccine
IS - 3
ER -