A low temperature microbial biosensor using immobilised psychrophilic bacteria

Renu Nandakumar, Bo Mattiasson

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A psychrophilic bacteria, Deinococcus radiodurans, was used to construct a biosensor to be used in a flow injection system. The transducer used was an O2 electrode. The response of this cell-based electrode was studied towards a number of sugars. The temperature dependence of the electrode response correlated well with the behavior of the cells. Thus, the optimum temperature for measurement of glucose (0.55 mM) was about +5 °C. Since the organism used is psychrophilic, a response time at this low temperature is similar to what is achieved with mesophilic organisms at room temperature. This is the first biosensor constructed using a psychrophilic microorganism.

Original languageEnglish (US)
Pages (from-to)689-693
Number of pages5
JournalBiotechnology Techniques
Volume13
Issue number10
DOIs
StatePublished - 1999

Keywords

  • Flow injection system
  • Low temperature
  • Microbial biosensor
  • Psychrophilic bacteria

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'A low temperature microbial biosensor using immobilised psychrophilic bacteria'. Together they form a unique fingerprint.

Cite this