A method for determination of antibody-dependent cellular cytotoxicity (ADCC) of human peripheral mononuclear cells

James Wisecarver; Thomas Bechtold; Michaeleen Collins; Jack Davis; Helen Lipscomb; Joseph Sonnabend; David T. Purtilo

doi:10.1016/0022-1759(85)90107-3

A method for determination of antibody-dependent cellular cytotoxicity (ADCC) of human peripheral mononuclear cells

James Wisecarver, Thomas Bechtold, Michaeleen Collins, Jack Davis, Helen Lipscomb, Joseph Sonnabend, David T. Purtilo

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A method is described for measuring antibody-dependent cellular cytotoxicity (ADCC) of mononuclear cells from human peripheral blood using an established murine cell line and commercially prepared antisera. The test utilizes a standard ⁵¹Cr release technique. The ADCC activity of mononuclear cells obtained from 10 healthy human volunteers was measured at 4 different effector: target cell ratios. A linear relationship between %⁵¹Cr release (ADCC) and the number of effector cells was observed.

Original language	English (US)
Pages (from-to)	277-282
Number of pages	6
Journal	Journal of Immunological Methods
Volume	79
Issue number	2
DOIs	https://doi.org/10.1016/0022-1759(85)90107-3
State	Published - May 23 1985

Keywords

antibody-dependent cellular cytotoxicity
cell-mediated cytotoxicity
human mononuclear cells

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0022-1759(85)90107-3

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title = "A method for determination of antibody-dependent cellular cytotoxicity (ADCC) of human peripheral mononuclear cells",

abstract = "A method is described for measuring antibody-dependent cellular cytotoxicity (ADCC) of mononuclear cells from human peripheral blood using an established murine cell line and commercially prepared antisera. The test utilizes a standard 51Cr release technique. The ADCC activity of mononuclear cells obtained from 10 healthy human volunteers was measured at 4 different effector: target cell ratios. A linear relationship between %51Cr release (ADCC) and the number of effector cells was observed.",

keywords = "antibody-dependent cellular cytotoxicity, cell-mediated cytotoxicity, human mononuclear cells",

author = "James Wisecarver and Thomas Bechtold and Michaeleen Collins and Jack Davis and Helen Lipscomb and Joseph Sonnabend and Purtilo, {David T.}",

note = "Funding Information: * This work was supported in part by PHS Grant no. 30196, awarded by the National Cancer Institute, PHHS, the Nebraska Department of Health, LB506, the Lymphoproliferative Research Fund, and the Book_meyer Research Funds. ** Correspondence to: David T. Purtilo, M.D., Department of Pathology and Laboratory Medicine, The University of Nebraska Medical Center, 42nd & Dewey Avenue, Omaha, NE 68105, U.S.A.",

year = "1985",

month = may,

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doi = "10.1016/0022-1759(85)90107-3",

language = "English (US)",

volume = "79",

pages = "277--282",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier B.V.",

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T1 - A method for determination of antibody-dependent cellular cytotoxicity (ADCC) of human peripheral mononuclear cells

AU - Wisecarver, James

AU - Bechtold, Thomas

AU - Collins, Michaeleen

AU - Davis, Jack

AU - Lipscomb, Helen

AU - Sonnabend, Joseph

AU - Purtilo, David T.

N1 - Funding Information: * This work was supported in part by PHS Grant no. 30196, awarded by the National Cancer Institute, PHHS, the Nebraska Department of Health, LB506, the Lymphoproliferative Research Fund, and the Book_meyer Research Funds. ** Correspondence to: David T. Purtilo, M.D., Department of Pathology and Laboratory Medicine, The University of Nebraska Medical Center, 42nd & Dewey Avenue, Omaha, NE 68105, U.S.A.

PY - 1985/5/23

Y1 - 1985/5/23

N2 - A method is described for measuring antibody-dependent cellular cytotoxicity (ADCC) of mononuclear cells from human peripheral blood using an established murine cell line and commercially prepared antisera. The test utilizes a standard 51Cr release technique. The ADCC activity of mononuclear cells obtained from 10 healthy human volunteers was measured at 4 different effector: target cell ratios. A linear relationship between %51Cr release (ADCC) and the number of effector cells was observed.

AB - A method is described for measuring antibody-dependent cellular cytotoxicity (ADCC) of mononuclear cells from human peripheral blood using an established murine cell line and commercially prepared antisera. The test utilizes a standard 51Cr release technique. The ADCC activity of mononuclear cells obtained from 10 healthy human volunteers was measured at 4 different effector: target cell ratios. A linear relationship between %51Cr release (ADCC) and the number of effector cells was observed.

KW - antibody-dependent cellular cytotoxicity

KW - cell-mediated cytotoxicity

KW - human mononuclear cells

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JF - Journal of Immunological Methods

IS - 2

ER -

A method for determination of antibody-dependent cellular cytotoxicity (ADCC) of human peripheral mononuclear cells

Abstract

Keywords

ASJC Scopus subject areas

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