A method for determination of antibody-dependent cellular cytotoxicity (ADCC) of human peripheral mononuclear cells

James Wisecarver, Thomas Bechtold, Michaeleen Collins, Jack Davis, Helen Lipscomb, Joseph Sonnabend, David T. Purtilo

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A method is described for measuring antibody-dependent cellular cytotoxicity (ADCC) of mononuclear cells from human peripheral blood using an established murine cell line and commercially prepared antisera. The test utilizes a standard 51Cr release technique. The ADCC activity of mononuclear cells obtained from 10 healthy human volunteers was measured at 4 different effector: target cell ratios. A linear relationship between %51Cr release (ADCC) and the number of effector cells was observed.

Original languageEnglish (US)
Pages (from-to)277-282
Number of pages6
JournalJournal of Immunological Methods
Volume79
Issue number2
DOIs
StatePublished - May 23 1985

Keywords

  • antibody-dependent cellular cytotoxicity
  • cell-mediated cytotoxicity
  • human mononuclear cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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