A method for extracting high-molecular-weight deoxyribonucleic acid from fungi

Charles A. Specht, Concetta C. DiRusso, Charles P. Novotny, Robert C. Ullrich

Research output: Contribution to journalArticle

162 Scopus citations

Abstract

A new method for isolating high-molecular-weight DNA from mycelium of the basidiomycete Schizophyllum commune is reported. Lyophilized mycelium is broken with mortar and pestle, and DNA extracted during gentle shaking in buffer containing 2% sodium dodecyl sulfate and toluene. DNA with double-strand length in excess of 80 kilobase pairs and singlestrand length of 36 kilobases can be prepared routinely in milligram quantities. This DNA is of high purity and suitable for reassociations and recombinant DNA studies. The procedure has also been used to prepare DNA from another filamentous basidiomycete, Heterobasidion annosum, and from yeast. With slight modification the procedure is attractive for isolating recombinant plasmids from yeast.

Original languageEnglish (US)
Pages (from-to)158-163
Number of pages6
JournalAnalytical Biochemistry
Volume119
Issue number1
DOIs
StatePublished - Jan 1 1982

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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