A microfluidic platform for investigating the impairment of mitochondrial transport

Hyung Joon Kim, Jeong Won Park, Joseph Harris, Behrad Vahidi, Noo Li Jeon

Research output: Contribution to conferencePaper

Abstract

This paper will describe a microfluidic platform that can be used to quantitatively evaluate axonal transport of mitochondria using live cell imaging. Primary rat cortical neurons were cultured and transfected with mito-GFP. We have successfully used this device to analyze the trafficking of fluorescently labeled mitochondria in the axons. Fluidically isolated application of A in the somal or axonal compartments resulted in considerable decrease in mitochondria movement in location dependent manner. Mitochondria trafficking slowed down more significantly proximal to location of A exposure. This paper demonstrates a promising application of microfluidics technology for investigating axonal transport related to Alzheimer's Disease.

Original languageEnglish (US)
Pages1147-1149
Number of pages3
StatePublished - 2008
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: Oct 12 2008Oct 16 2008

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period10/12/0810/16/08

Keywords

  • Abeta
  • Microfluidics
  • Mitochondrial transport
  • Neurons

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Kim, H. J., Won Park, J., Harris, J., Vahidi, B., & Li Jeon, N. (2008). A microfluidic platform for investigating the impairment of mitochondrial transport. 1147-1149. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.