Abstract
This paper will describe a microfluidic platform that can be used to quantitatively evaluate axonal transport of mitochondria using live cell imaging. Primary rat cortical neurons were cultured and transfected with mito-GFP. We have successfully used this device to analyze the trafficking of fluorescently labeled mitochondria in the axons. Fluidically isolated application of A in the somal or axonal compartments resulted in considerable decrease in mitochondria movement in location dependent manner. Mitochondria trafficking slowed down more significantly proximal to location of A exposure. This paper demonstrates a promising application of microfluidics technology for investigating axonal transport related to Alzheimer's Disease.
| Original language | English (US) |
|---|---|
| Pages | 1147-1149 |
| Number of pages | 3 |
| State | Published - 2008 |
| Externally published | Yes |
| Event | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008 |
Other
| Other | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 |
|---|---|
| Country/Territory | United States |
| City | San Diego, CA |
| Period | 10/12/08 → 10/16/08 |
Keywords
- Abeta
- Microfluidics
- Mitochondrial transport
- Neurons
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering