A microplate immunofluorescence assay for the detection of monoclonal antibodies against viral antigens in cell culture

D. V. Onisk, S. Srikumaran, C. L. Kelling

Research output: Contribution to journalArticle

3 Scopus citations


A microplate immunofluorescence assay for the screening of culture fluids from large numbers of hybridoma clones has been developed. This method enables the simultaneous screening of a small volume of culture fluid from initial hybridoma clones on several different viruses or several strains of the same virus, enabling the early isolation and stabilization of the clones with the desired specificity.

Original languageEnglish (US)
Pages (from-to)203-206
Number of pages4
JournalJournal of Immunological Methods
Issue number1-2
StatePublished - Dec 20 1989



  • Hybridoma
  • Immunofluorescence
  • Monoclonal antibody
  • Viral antigen

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this