A model for the dynamic nuclear/nucleolar/cytoplasmic trafficking of the porcine reproductive and respiratory syndrome virus (PRRSV) nucleocapsid protein based on live cell imaging

Jae Hwan You, Gareth Howell, Asit K. Pattnaik, Fernando A. Osorio, Julian A. Hiscox

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV), an arterivirus, in common with many other positive strand RNA viruses, encodes a nucleocapsid (N) protein which can localise not only to the cytoplasm but also to the nucleolus in virus-infected cells and cells over-expressing N protein. The dynamic trafficking of positive strand RNA virus nucleocapsid proteins and PRRSV N protein in particular between the cytoplasm and nucleolus is unknown. In this study live imaging of permissive and non-permissive cell lines, in conjunction with photo-bleaching (FRAP and FLIP), was used to investigate the trafficking of fluorescent labeled (EGFP) PRRSV-N protein. The data indicated that EGFP-PRRSV-N protein was not permanently sequestered to the nucleolus and had equivalent mobility to cellular nucleolar proteins. Further the nuclear import of N protein appeared to occur faster than nuclear export, which may account for the observed relative distribution of N protein between the cytoplasm and the nucleolus.

Original languageEnglish (US)
Pages (from-to)34-47
Number of pages14
JournalVirology
Volume378
Issue number1
DOIs
StatePublished - Aug 15 2008

Keywords

  • Arterivirus
  • Confocal
  • Cytoplasm
  • FLIP
  • FRAP
  • Nuclear export
  • Nuclear import
  • Nucleocapsid protein
  • Nucleolar
  • Nucleolus
  • Nucleus
  • PRRSV
  • Virus

ASJC Scopus subject areas

  • Virology

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