A monoclonal antibody sandwich immunoassay for serum amyloid A (SAA) protein

Thomas L. McDonald, Annika Weber, James W. Smith

Research output: Contribution to journalArticlepeer-review

71 Scopus citations


An antibody sandwich immunoassay using two purified rat monoclonal antibodies to human serum amyloid A was developed and used to measure serum amyloid A in human serum. The assay was specific, sensitive, reproducible, and reliable and does not require denaturation of the specimen prior to assay. Serum amyloid A purified by hydrophobic interaction chromatography of acute phase human serum afforded a reliable standard for the assay. A significant (r = 0.69) correlation for SAA and C reactive protein values was found for 180 patient samples analyzed.

Original languageEnglish (US)
Pages (from-to)149-155
Number of pages7
JournalJournal of Immunological Methods
Issue number2
StatePublished - Nov 22 1991


  • C reactive protein
  • Monoclonal antibody
  • Serum amyloid A

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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