Collecting plant phenotypic data with sufficient resolution (in both space and time) and accuracy represents a long standing challenge in plant science research, and has been a major limiting factor for the effective use of genomic data for crop improvement. This is particularly true in plant breeding where collecting large-scale field-based plant phenotypes can be very labor intensive and costly. In this paper we reported a multi-sensor system for high throughput phenotyping in plant breeding. The system comprised five sensor modules (ultrasonic distance sensors, thermal infrared radiometers, NDVI sensors, portable spectrometers, and RGB web cameras) to measure crop canopy traits from field plots. A GPS was used to geo-reference the sensor measurements. Two environmental sensors (a solar radiation sensor and air temperature/relative humidity sensor) were also integrated into the system to collect simultaneous environmental data. A LabVIEW program was developed to control and synchronize measurements from all sensor modules and stored sensor readings in the host computer. Canopy reflectance spectra (by portable spectrometers) were post processed to extract NDVI and red-edge NDVI spectral indices; and RGB images were post processed to extract canopy green pixel fraction (as a proxy for biomass). The sensor system was tested in a soybean and wheat field trial. The results showed strong correlations among the sensor-based plant traits at both early and late growing season. Significant correlations were also found between the sensor-based traits and final grain yield at the early season (Pearson's correlation coefficient r ranged from 0.41 to 0.55) and late season (r from 0.55 to 0.70), suggesting the potential use of the sensor system to assist in phenotypic selection for plant breeding. The sensor system performed satisfactorily and robustly in the field tests. It was concluded that the sensor system could be a powerful tool for plant breeders to collect field-based, high throughput plant phenotyping data.
- Canopy reflectance
- Canopy temperature
- High throughput field phenotyping
- RGB image
ASJC Scopus subject areas
- Agronomy and Crop Science
- Computer Science Applications