A multi-step NMR screen for the identification and evaluation of chemical leads for drug discovery

Kelly A. Mercier, Matthew D. Shortridge, Robert Powers

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

A multi-step NMR based screening assay is described for identifying and evaluating chemical leads for their ability to bind a target protein. The multi-step NMR assay provides structure-related information while being an integral part of a structure based drug discovery and design program. The fundamental principle of the multi-step NMR assay is to combine distinct 1D and 2D NMR techniques, in such a manner, that the inherent strengths and weakness associated with each technique is complementary to each other in the screen. By taking advantage of the combined strengths of 1D and 2D NMR experiments, it is possible to minimize protein requirements and experiment time and differentiate between nonspecific and stoichiometric binders while being able to verify ligand binding, determine a semi-quantitative dissociation constant, identify the ligand binding site and rapidly determine a protein-ligand co-structure. Furthermore, the quality and physical behavior of the ligand is readily evaluated to determine its appropriateness as a chemical lead. The utility of the multi-step NMR assay is demonstrated with the use of PrgI from Salmonella typhimurium and human serum albumin (HSA) as target proteins.

Original languageEnglish (US)
Pages (from-to)285-295
Number of pages11
JournalCombinatorial Chemistry and High Throughput Screening
Volume12
Issue number3
DOIs
StatePublished - Mar 1 2009

Keywords

  • 2D 1H-15N HSQC
  • Human serum albumin
  • Line broadening
  • Multi-step NMR screen
  • Protein PrgI
  • Protein-ligand binding
  • Salmonella typhimurium
  • Saturation transfer difference

ASJC Scopus subject areas

  • Drug Discovery
  • Computer Science Applications
  • Organic Chemistry

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