A multiplex reverse transcription-polymerase chain reaction assay for newcastle disease virus and avian pneumovirus (colorado strain)

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Newcastle disease virus (NDV) and avian pneumovirus (APV) cause Newcastle disease and rhinotracheitis respectively, in turkeys. Both of these viruses infect the respiratory system. A one-tube, multiplex, reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of both NDV and Colorado strain of APV (APV-Col) was developed and evaluated. The primers, specific for each virus, were designed from the matrix protein gene of APV-Col and the fusion protein gene of NDV to amplify products of 631 and 309 nucleotides, respectively. The multiplex RT-PCR assay, for detecting both viruses simultaneously, was compared with the single-virus RT-PCR assays for its sensitivity and specificity. The specific primers amplified products of predicted size from each virus in the multiplex as well as the single-virus RT-PCR assays. The multiplex RT-PCR assay was determined to be equivalent to the single-virus RT-PCR assays for detecting both NDV and APV-Col. This multiplex RT-PCR assay proved to be a sensitive method for the simultaneous and rapid detection of NDV and APV-Col. This assay has the potential for clinical diagnostic applications.

Original languageEnglish (US)
Pages (from-to)938-943
Number of pages6
JournalAvian diseases
Volume44
Issue number4
DOIs
StatePublished - 2000
Externally publishedYes

Keywords

  • Avian pneumovirus
  • Multiplex RT-PCR
  • Newcastle disease
  • Newcastle disease virus
  • RT-PCR
  • Turkey rhinotracheitis

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

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