A new blood collection device minimizes cellular DNA release during sample storage and shipping when compared to a standard device

Sheila E. Norton, Kristin K. Luna, Joel M. Lechner, Jianbing Qin, M. Rohan Fernando

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Background: Cell-free DNA (cfDNA) circulating in blood is currently used for noninvasive diagnostic and prognostic tests. Minimizing background DNA is vital for detection of low abundance cfDNA. We investigated whether a new blood collection device could reduce background levels of genomic DNA (gDNA) in plasma compared to K3EDTA tubes, when subjected to conditions that may occur during sample storage and shipping. Methods: Blood samples were drawn from healthy donors into K3EDTA and Cell-Free DNA™ BCT (BCT). To simulate shipping, samples were shaken or left unshaken. In a shipping study, samples were shipped or not shipped. To assess temperature variations, samples were incubated at 6°C, 22°C, and 37°C. In all cases, plasma was harvested by centrifugation and total plasma DNA (pDNA) assayed by quantitative real-time polymerase chain reaction (qPCR). Results: Shaking and shipping blood in K3EDTA tubes showed significant increases in pDNA, whereas no change was seen in BCTs. Blood in K3EDTA tubes incubated at 6°C, 22°C, and 37°C showed increases in pDNA while pDNA from BCTs remained stable. Conclusions: BCTs prevent increases in gDNA levels that can occur during sample storage and shipping. This new device permits low abundance DNA target detection and allows accurate cfDNA concentrations.

Original languageEnglish (US)
Pages (from-to)305-311
Number of pages7
JournalJournal of Clinical Laboratory Analysis
Volume27
Issue number4
DOIs
StatePublished - Jul 1 2013

Keywords

  • Blood preservation
  • Clinical laboratory techniques
  • Hematology
  • Investigative techniques
  • Real-time polymerase chain reaction

ASJC Scopus subject areas

  • Immunology and Allergy
  • Hematology
  • Public Health, Environmental and Occupational Health
  • Clinical Biochemistry
  • Medical Laboratory Technology
  • Biochemistry, medical
  • Microbiology (medical)

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