A new class of antivirals: antisense oligonucleotides combined with a hydrophobic substituent effectively inhibit influenza virus reproduction and synthesis of virus-specific proteins in MDCK cells

Alexander V. Kabanov; Sergei V. Vinogradov; Alexander V. Ovcharenko; Alexander V. Krivonos; Nikolai S. Melik-Nubarov; Vsevolod I. Kiselev; Eugenii S. Severin

doi:10.1016/0014-5793(90)80039-L

A new class of antivirals: antisense oligonucleotides combined with a hydrophobic substituent effectively inhibit influenza virus reproduction and synthesis of virus-specific proteins in MDCK cells

Alexander V. Kabanov, Sergei V. Vinogradov, Alexander V. Ovcharenko, Alexander V. Krivonos, Nikolai S. Melik-Nubarov, Vsevolod I. Kiselev, Eugenii S. Severin

Research output: Contribution to journal › Article › peer-review

96 Scopus citations

Abstract

To enhance the penetration of oligonucleotide ('oligo') into cells, the oligo was combined with the hydrophobic undecyl residue. Using the 'DNA-synthesator', we synthesized oligo, complementary to the loop-forming site of the RNA, encoding polymerase 3 of the influenza virus (type A), and combined it with the undecyl residue added to the 5' terminal phosphate group. It was found that the modified oligo effectively suppresses the influenza A/PR8/34 (H1N1) virus reproduction and inhibits the synthesis of virus-specific proteins in MDCK cells. Under the same conditions, the non-modified antisense oligo and modified nonsense oligo did not affect the virus development.

Original language	English (US)
Pages (from-to)	327-330
Number of pages	4
Journal	FEBS Letters
Volume	259
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(90)80039-L
State	Published - Jan 1 1990
Externally published	Yes

Keywords

Antisense
Influenza
Inhibition
Oligonucleotide
Translation
Virus

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(90)80039-L

Cite this

Kabanov, A. V., Vinogradov, S. V., Ovcharenko, A. V., Krivonos, A. V., Melik-Nubarov, N. S., Kiselev, V. I., & Severin, E. S. (1990). A new class of antivirals: antisense oligonucleotides combined with a hydrophobic substituent effectively inhibit influenza virus reproduction and synthesis of virus-specific proteins in MDCK cells. FEBS Letters, 259(2), 327-330. https://doi.org/10.1016/0014-5793(90)80039-L

A new class of antivirals: antisense oligonucleotides combined with a hydrophobic substituent effectively inhibit influenza virus reproduction and synthesis of virus-specific proteins in MDCK cells. / Kabanov, Alexander V.; Vinogradov, Sergei V.; Ovcharenko, Alexander V. et al.
In: FEBS Letters, Vol. 259, No. 2, 01.01.1990, p. 327-330.

Research output: Contribution to journal › Article › peer-review

Kabanov, AV, Vinogradov, SV, Ovcharenko, AV, Krivonos, AV, Melik-Nubarov, NS, Kiselev, VI & Severin, ES 1990, 'A new class of antivirals: antisense oligonucleotides combined with a hydrophobic substituent effectively inhibit influenza virus reproduction and synthesis of virus-specific proteins in MDCK cells', FEBS Letters, vol. 259, no. 2, pp. 327-330. https://doi.org/10.1016/0014-5793(90)80039-L

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abstract = "To enhance the penetration of oligonucleotide ('oligo') into cells, the oligo was combined with the hydrophobic undecyl residue. Using the 'DNA-synthesator', we synthesized oligo, complementary to the loop-forming site of the RNA, encoding polymerase 3 of the influenza virus (type A), and combined it with the undecyl residue added to the 5' terminal phosphate group. It was found that the modified oligo effectively suppresses the influenza A/PR8/34 (H1N1) virus reproduction and inhibits the synthesis of virus-specific proteins in MDCK cells. Under the same conditions, the non-modified antisense oligo and modified nonsense oligo did not affect the virus development.",

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A new class of antivirals: antisense oligonucleotides combined with a hydrophobic substituent effectively inhibit influenza virus reproduction and synthesis of virus-specific proteins in MDCK cells

Abstract

Keywords

ASJC Scopus subject areas

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