A novel ABA-dependent dehydrin ERD10 gene from Brassica napus

Zhongxiang Deng, Yongzhen Pang, Weiwen Kong, Zhonghai Chen, Xinglong Wang, Xiaojun Liu, Yan Pi, Xiaofen Sun, Kexuan Tang

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

A new dehydrin ERD10 gene was cloned and characterized from Brassica napus (designated as Bndhn ERD10). The full-length cDNA of Bndhn ERD10 was 1114 bp and contained an open reading frame of 816 bp encoding a protein of 271 amino acid residues. The deduced Bndhn ERD10 protein contained an 8-serine residue domain and two conserved repeats of the characterized lysine-rich-K-segment (KIKEKLPG). Analysis of full-length cDNA and genomic DNA indicated that there were no introns in Bndhn ERD10 gene. The promoter of Bndhn ERD10 was further obtained by genomic walking technology, and analysis of the promoter indicated that the regulation of Bndhn ERD10 was ABA-dependent. Semi-quantitative RT-PCR of different tissues in unstressed B. napus plants indicated that the transcript of Bndhn ERD10 was more abundant in leaf than in stem and root. The expression profiles of Bndhn ERD10 in B. napus seedlings under various stress conditions including cold, salt and ABA were also investigated. Upon cold, salt and ABA stresses, increased transcript accumulations of the Bndhn ERD10 mRNAs were detected in young leaves 8 h after treatment.

Original languageEnglish (US)
Pages (from-to)28-35
Number of pages8
JournalDNA Sequence - Journal of DNA Sequencing and Mapping
Volume16
Issue number1
DOIs
StatePublished - Feb 2005
Externally publishedYes

Keywords

  • ABA
  • Bndhn ERD10
  • Brassica napus
  • Dehydrin
  • RACE

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Endocrinology

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