A novel adjuvant for vaccine development in the aged

Edward L. Morgan, Marilyn L. Thoman, Sam D. Sanderson, Joy A. Phillips

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

A conformationally-biased, response-selective agonist of human C5a65-74 (EP67) activated antigen presenting cells (APC) from aged C57Bl/6 mice in vitro and the generation of antigen (Ag)-specific antibody (Ab) responses in aged mice in vivo. EP67, induced the release of the pro-inflammatory cytokines IL-6, TNFα, and INFγ from splenic APCs obtained from both aged and young mice. Both aged and young mice produced high Ag-specific IgG Ab titers when immunized with EP67-containing vaccines to ovalbumin (OVA-EP67) and to a protein (rPrp1) from the cell wall of Coccidioides (rPrp1-EP67). Immunization with EP67-containing vaccines resulted in higher IgG titers in both young and aged mice compared to mice immunized with OVA adsorbed to alum (OVA/alum) and Prp1 admixed with CpG (rPrp1 +CpG). Aged and young mice immunized with the EP67-containing vaccines generated higher titers of IgG1 and IgG2b relative to their aged-matched counterparts immunized with OVA/alum or Prp1 +CpG. These results indicate that EP67 induces humoral immunity in aged mice not obtainable with alum and CpG. These results support the use of EP67 as a potential vaccine adjuvant suited to the elderly.

Original languageEnglish (US)
Pages (from-to)8275-8279
Number of pages5
JournalVaccine
Volume28
Issue number52
DOIs
StatePublished - Dec 6 2010
Externally publishedYes

Keywords

  • Adjuvant
  • Aging
  • Antibody response responses
  • Cytokines
  • Immunization
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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  • Cite this

    Morgan, E. L., Thoman, M. L., Sanderson, S. D., & Phillips, J. A. (2010). A novel adjuvant for vaccine development in the aged. Vaccine, 28(52), 8275-8279. https://doi.org/10.1016/j.vaccine.2010.10.008