A novel RNA helicase gene tightly linked to the Triplolethal locus of Drosophila

Douglas R. Dorer; Alan C. Christensen; Daniel H. Johnson

doi:10.1093/nar/18.18.5489

A novel RNA helicase gene tightly linked to the Triplolethal locus of Drosophila

Douglas R. Dorer, Alan C. Christensen, Daniel H. Johnson

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

The Triplo-lethal (Tpl) locus of Drosophila is the only known locus which is lethal when present in three copies rather than the normal two. After recovering a hybrid-dysgenesis-induced mutation of Tpl we used a rapid combination of transposon tagging, chromosome microdissection and PCR to clone the P element that had transposed into the Tpl region. That P element is located within the gene for a new and unique member of the RNA helicase family. This new helicase differs from all others known by having glycine-rich repeats at both the amino and carboxyl termini. Curiously, genetic analysis shows that the P element inserted into this gene is not responsible for the Tpl mutant phenotype. We present possible explanations for these findings.

Original language	English (US)
Pages (from-to)	5489-5494
Number of pages	6
Journal	Nucleic acids research
Volume	18
Issue number	18
DOIs	https://doi.org/10.1093/nar/18.18.5489
State	Published - Sep 25 1990
Externally published	Yes

ASJC Scopus subject areas

Genetics

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10.1093/nar/18.18.5489

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title = "A novel RNA helicase gene tightly linked to the Triplolethal locus of Drosophila",

abstract = "The Triplo-lethal (Tpl) locus of Drosophila is the only known locus which is lethal when present in three copies rather than the normal two. After recovering a hybrid-dysgenesis-induced mutation of Tpl we used a rapid combination of transposon tagging, chromosome microdissection and PCR to clone the P element that had transposed into the Tpl region. That P element is located within the gene for a new and unique member of the RNA helicase family. This new helicase differs from all others known by having glycine-rich repeats at both the amino and carboxyl termini. Curiously, genetic analysis shows that the P element inserted into this gene is not responsible for the Tpl mutant phenotype. We present possible explanations for these findings.",

author = "Dorer, {Douglas R.} and Christensen, {Alan C.} and Johnson, {Daniel H.}",

note = "Funding Information: We are grateful to Marilyn Cadden and Helene Klapper for skilled technical assistance, to Jonathan Schiffman for isolating the TpP34revertants, to Tom Kornberg for the cDNA libraries, to Mark Champe and Charles Laird for the cosmid library, and to Steve Henikoff for pointing out the vertical DEAD box at position 146. This work was supported in part by grants from the National Institutes of Health to A.C.C. (GM38483) and D.H.J. (HG00081), and a predoctoral fellowship to D.R.D. from the Percival E. and Ethel Brown Foerderer Foundation.",

year = "1990",

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doi = "10.1093/nar/18.18.5489",

language = "English (US)",

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T1 - A novel RNA helicase gene tightly linked to the Triplolethal locus of Drosophila

AU - Dorer, Douglas R.

AU - Christensen, Alan C.

AU - Johnson, Daniel H.

N1 - Funding Information: We are grateful to Marilyn Cadden and Helene Klapper for skilled technical assistance, to Jonathan Schiffman for isolating the TpP34revertants, to Tom Kornberg for the cDNA libraries, to Mark Champe and Charles Laird for the cosmid library, and to Steve Henikoff for pointing out the vertical DEAD box at position 146. This work was supported in part by grants from the National Institutes of Health to A.C.C. (GM38483) and D.H.J. (HG00081), and a predoctoral fellowship to D.R.D. from the Percival E. and Ethel Brown Foerderer Foundation.

PY - 1990/9/25

Y1 - 1990/9/25

N2 - The Triplo-lethal (Tpl) locus of Drosophila is the only known locus which is lethal when present in three copies rather than the normal two. After recovering a hybrid-dysgenesis-induced mutation of Tpl we used a rapid combination of transposon tagging, chromosome microdissection and PCR to clone the P element that had transposed into the Tpl region. That P element is located within the gene for a new and unique member of the RNA helicase family. This new helicase differs from all others known by having glycine-rich repeats at both the amino and carboxyl termini. Curiously, genetic analysis shows that the P element inserted into this gene is not responsible for the Tpl mutant phenotype. We present possible explanations for these findings.

AB - The Triplo-lethal (Tpl) locus of Drosophila is the only known locus which is lethal when present in three copies rather than the normal two. After recovering a hybrid-dysgenesis-induced mutation of Tpl we used a rapid combination of transposon tagging, chromosome microdissection and PCR to clone the P element that had transposed into the Tpl region. That P element is located within the gene for a new and unique member of the RNA helicase family. This new helicase differs from all others known by having glycine-rich repeats at both the amino and carboxyl termini. Curiously, genetic analysis shows that the P element inserted into this gene is not responsible for the Tpl mutant phenotype. We present possible explanations for these findings.

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A novel RNA helicase gene tightly linked to the Triplolethal locus of Drosophila

Abstract

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