A novel role for proteomics in the discovery of cell-surface markers on stem cells: Scratching the surface

Rebekah L. Gundry, Kenneth R. Boheler, Jennifer E. Van Eyk, Bernd Wollscheid

Research output: Contribution to journalReview article

31 Scopus citations

Abstract

The concept of cell-based therapy has been advocated as a novel approach for treating diseases or conditions where regeneration of cells, tissue and/or potentially organs is required. A promising source for cell-replacement therapies is provided by stem cells, but the success of this approach will ultimately rely on the ability to isolate primary stem or progenitor cells. Cell-surface protein markers will play a critical role in this step. Current methodologies for the identification of cell-surface protein markers rely primarily on antibody availability and flow cytometry, but many cell-surface proteins remain undetectable. Proteomic technologies now offer the possibility to specifically identify and investigate the cell-surface subproteome in a quantitative and discovery-driven manner. Once a cell surface protein marker panel has been identified by MS and the antibodies become available, the panel should permit the identification, tracking, and/or isolation of stem or progenitor cells that may be appropriate for therapeutics. This review provides a context for the use of proteomics in discovering new cell-surface markers for stem cells.

Original languageEnglish (US)
Pages (from-to)892-903
Number of pages12
JournalProteomics - Clinical Applications
Volume2
Issue number6
DOIs
StatePublished - Jun 2008

Keywords

  • Cell surface protein marker
  • Stem cells

ASJC Scopus subject areas

  • Clinical Biochemistry

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