A novel ultrasensitive LC-MS/MS assay for quantification of intracellular raltegravir in human cell extracts

Brian L. Robbins, Sarah R. Nelson, Courtney V. Fletcher

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

An assay using ultrahigh pressure liquid chromatography and mass spectrometry detection was developed and validated for measurement of the HIV integrase inhibitor raltegravir (MK-0518) in human cell extracts. The assay is designed to utilize 200μl of 70% MeOH cell extract derived from human peripheral blood mononuclear cells or human tissue samples. The assay is linear over a range from 0.0023 to 9.2ngml-1. The average %CV (SD/Mean)100 and %deviation ((observed-target)/target)100 were less than 20% at the lower limit of quantification and less than 15% over the range of the curve. This assay is an accurate and highly sensitive method for determining raltegravir concentrations in cellular extracts with a lower limit 40 to over 100-fold lower than other methods in the literature. We also present a new processing method where a rapid spin through oil produced a significant increase in apparent intracellular raltegravir concentration compared with conventional processing.

Original languageEnglish (US)
Pages (from-to)378-387
Number of pages10
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume70
DOIs
StatePublished - Nov 2012

Keywords

  • HIV
  • Integrase inhibitor
  • Intracellular
  • LC-MS/MS
  • Raltegravir

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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