A permease-like protein involved in ER to thylakoid lipid transfer in Arabidopsis

Changcheng Xu, Jilian Fan, Wayne Riekhof, John E. Froehlich, Christoph Benning

Research output: Contribution to journalArticle

147 Scopus citations

Abstract

In eukaryotes, enzymes of different subcellular compartments participate in the assembly of membrane lipids. As a consequence, interorganelle lipid transfer is extensive in growing cells. A prominent example is the transfer of membrane lipid precursors between the endoplasmic reticulum (ER) and the photosynthetic thylakoid membranes in plants. Mono- and digalactolipids are typical photosynthetic membrane lipids. In Arabidopsis, they are derived from one of two pathways, either synthesized de novo in the plastid, or precursors are imported from the ER, giving rise to distinct molecular species. Employing a high-throughput robotic screening procedure generating arrays of spot chromatograms, mutants of Arabidopsis were isolated, which accumulated unusual trigalactolipids. In one allelic mutant subclass, trigalactosyldiacylglycerol1, the primary defect caused a disruption in the biosynthesis of ER-derived thylakoid lipids. Secondarily, a processive galactosyltransferase was activated, leading to the accumulation of oligogalactolipids. Mutations in a permease-like protein of the outer chloroplastic envelope are responsible for the primary biochemical defect. It is proposed that this protein is part of a lipid transfer complex.

Original languageEnglish (US)
Pages (from-to)2370-2379
Number of pages10
JournalEMBO Journal
Volume22
Issue number10
DOIs
StatePublished - May 15 2003
Externally publishedYes

Keywords

  • Chloroplast envelopes
  • Lipid arrays
  • Lipid permease
  • Lipid trafficking
  • Thylakoid lipids

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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