A photoactivatable Src homology 2 (SH2) domain

X. Song, X. Shang, T. Ju, R. Cerny, W. Niu, J. Guo

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Src homology 2 (SH2) domains bind specifically to phosphotyrosine-containing motifs. As a regulatory module of intracellular signaling cascades, the SH2 domain plays important roles in the signal transduction of receptor tyrosine kinase pathways. In this work, we reported the construction of a photoactivatable SH2 domain through the combination of protein engineering and genetic incorporation of photo-caged unnatural amino acids. Significantly enhanced recognition of a phosphotyrosine-containing peptide substrate by the engineered SH2 domain mutant was observed after light-induced removal of the photo-caging group. Optical activation allows the control of protein and/or cellular function with temporal and spatial resolution. This photoactivatable SH2 domain could potentially be applied to the study of tyrosine phsophorylation-associated biological processes.

Original languageEnglish (US)
Pages (from-to)51120-51124
Number of pages5
JournalRSC Advances
Issue number56
StatePublished - 2016

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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