A physical linkage map of HLA-A, -G, -7.5p, and -F

Cynthia M. Schmidt, Harry T. Orr

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

The class I region of the human leukocyte antigen (HLA) complex includes genes encoding the classical transplantation antigens (HLA-A, -B, -C), at least three nonclassical class I genes (HLA-E, -F, and -G), and many class I pseudogenes (including HLA-7.5p). We have used probes from DNA within or flanking the HLA -A, -F, -G, and -7.5p genes to construct a physical linkage map that places the HLA-F, -G, and -7.5p loci in order with respect to HLA-A. The map was constructed using clamped homogeneous electric field pulsed-field gel electrophoresis. DNA was isolated from LCL 721 (A1:B8, A2:B5), a human Epstein-Barr virus-transformed lymphoblastoid cell line (LCL), and from two γ-irradiation-induced mutants of LCL 721 lacking complementary class I haplotypes. The physical linkage data place HLA-G closest to HLA-A and place HLA-7.5p between HLA-G and HLA-F. The map constructed supports a maximum distance of 490 kilobases between HLA-A and HLA-F. Human Immunology 31, 180-185 (1991).

Original languageEnglish (US)
Pages (from-to)180-185
Number of pages6
JournalHuman Immunology
Volume31
Issue number3
DOIs
StatePublished - 1991

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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