TY - JOUR
T1 - A physical linkage map of HLA-A, -G, -7.5p, and -F
AU - Schmidt, Cynthia M.
AU - Orr, Harry T.
N1 - Funding Information:
We thank S.K. Bronson and Dr. D.D. Chaplin fi~r helpful discussions of unpublished data. We also thank Dr. S. Scherer and members of his laboratory for providing yeast cultures and protocols for the preparation of yeast chromosomes in agarose blocks, and Dr. J. Perentisis fi}r assisting in the identification of the largest S. cervisiae chromosomes. Support for these studies was provided by Grant AI 18124 from the N IH.
PY - 1991
Y1 - 1991
N2 - The class I region of the human leukocyte antigen (HLA) complex includes genes encoding the classical transplantation antigens (HLA-A, -B, -C), at least three nonclassical class I genes (HLA-E, -F, and -G), and many class I pseudogenes (including HLA-7.5p). We have used probes from DNA within or flanking the HLA -A, -F, -G, and -7.5p genes to construct a physical linkage map that places the HLA-F, -G, and -7.5p loci in order with respect to HLA-A. The map was constructed using clamped homogeneous electric field pulsed-field gel electrophoresis. DNA was isolated from LCL 721 (A1:B8, A2:B5), a human Epstein-Barr virus-transformed lymphoblastoid cell line (LCL), and from two γ-irradiation-induced mutants of LCL 721 lacking complementary class I haplotypes. The physical linkage data place HLA-G closest to HLA-A and place HLA-7.5p between HLA-G and HLA-F. The map constructed supports a maximum distance of 490 kilobases between HLA-A and HLA-F. Human Immunology 31, 180-185 (1991).
AB - The class I region of the human leukocyte antigen (HLA) complex includes genes encoding the classical transplantation antigens (HLA-A, -B, -C), at least three nonclassical class I genes (HLA-E, -F, and -G), and many class I pseudogenes (including HLA-7.5p). We have used probes from DNA within or flanking the HLA -A, -F, -G, and -7.5p genes to construct a physical linkage map that places the HLA-F, -G, and -7.5p loci in order with respect to HLA-A. The map was constructed using clamped homogeneous electric field pulsed-field gel electrophoresis. DNA was isolated from LCL 721 (A1:B8, A2:B5), a human Epstein-Barr virus-transformed lymphoblastoid cell line (LCL), and from two γ-irradiation-induced mutants of LCL 721 lacking complementary class I haplotypes. The physical linkage data place HLA-G closest to HLA-A and place HLA-7.5p between HLA-G and HLA-F. The map constructed supports a maximum distance of 490 kilobases between HLA-A and HLA-F. Human Immunology 31, 180-185 (1991).
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U2 - 10.1016/0198-8859(91)90024-4
DO - 10.1016/0198-8859(91)90024-4
M3 - Article
C2 - 1890019
AN - SCOPUS:0026040731
VL - 31
SP - 180
EP - 185
JO - Human Immunology
JF - Human Immunology
SN - 0198-8859
IS - 3
ER -