Little is known about the nature of Ag recognition by the TCR-γδ. The recent observation that γδ T cells preferentially recognize mycobacterial Ag provides a model to examine the molecular basis of γδ-TCR recognition. Here, examination of the Mycobacteria-stimulated peripheral blood T cells with TCR-specific mAb revealed a predominance of T cells bearing Vγ2/Vδ2 gene products. PCR cloning and sequence analysis of the TCR chains demonstrated extensive junctional diversity indicating that the response was polyclonal. The marked in vitro γδ T cell response to Mycobacteria was also detected in newborns before encounters with foreign Ag and exclusively involved the same V-gene usage observed in adults. Together, these results suggest that a major mechanism of γδ T cell reactivity involves recognition mediated by germline-encoded segments of the TCR.
|Original language||English (US)|
|Number of pages||10|
|Journal||Journal of Immunology|
|State||Published - 1991|
ASJC Scopus subject areas
- Immunology and Allergy