A protein fraction stably linked to DNA in plant chromatin

Zova Avramova, Maria Ivanchenko, Roumen Tsanev

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

DNA from the chromatin of roots and shoots of maize seedlings was isolated and extensively deproteinized by repeated high-salt extractions, by subsequent deproteinizations eliminating noncovalently associated proteins and by CsC1 density gradient centrifugation. Nevertheless, a protein component resisting all extraction procedures was found firmly associated to plant nuclear DNA. This component was responsible for the 125I uptake when a DNA preparation had been labeled by the chloramine-T method. A residual oligodeoxynucleotide-oligopeptide complex was obtained after extensive digestions of the initial DNA-protein complex with proteases and nucleases. The stability of this complex to different chemical treatments suggested a phosphoester type of a linkage. The hydrolysis of this complex by phosphodiesterases indicated that the protein component was linked to plant chromosomal DNA through a phosphodiester bond formed by a hydroxyaminoacid and a 5′-end DNA phosphate. Two-dimensional tryptic peptide mapping of the proteins isolated from the two maize chromatins revealed a high degree of similarity to the corresponding proteins of animal origin. Its conservative structure suggests an important role for this protein component in the functioning of the eukaryotic genome.

Original languageEnglish (US)
Pages (from-to)401-408
Number of pages8
JournalPlant Molecular Biology
Volume11
Issue number4
DOIs
StatePublished - Jul 1988
Externally publishedYes

Keywords

  • maize chromatin
  • stable DNA-protein complexes

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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