A rapid procedure for purification of EcoRI endonuclease

János Sümegi, Danielle Breedveld, paul Hossenlopp, Pierre Chambon

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

A convenient and rapid procedure has been developed to purify restriction endonuclease Eco RI. The method involves sonication of cells at low ionic strength, precipitation of the endonuclease with Polymin P (a polyethyleneimine), elution of the enzyme from the Polymin P precipitate, ammonium sulfate precipitation and chromatography on phosphocellulose. The purified restriction endonuclease is free of exonuclease and other endonucleases.

Original languageEnglish (US)
Pages (from-to)78-85
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume76
Issue number1
DOIs
StatePublished - May 9 1977

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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