Abstract
A convenient and rapid procedure has been developed to purify restriction endonuclease Eco RI. The method involves sonication of cells at low ionic strength, precipitation of the endonuclease with Polymin P (a polyethyleneimine), elution of the enzyme from the Polymin P precipitate, ammonium sulfate precipitation and chromatography on phosphocellulose. The purified restriction endonuclease is free of exonuclease and other endonucleases.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 78-85 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 76 |
| Issue number | 1 |
| DOIs | |
| State | Published - May 9 1977 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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Cite this
Sümegi, J., Breedveld, D., Hossenlopp, P., & Chambon, P. (1977). A rapid procedure for purification of EcoRI endonuclease. Biochemical and Biophysical Research Communications, 76(1), 78-85. https://doi.org/10.1016/0006-291X(77)91670-9