Abstract
A convenient and rapid procedure has been developed to purify restriction endonuclease Eco RI. The method involves sonication of cells at low ionic strength, precipitation of the endonuclease with Polymin P (a polyethyleneimine), elution of the enzyme from the Polymin P precipitate, ammonium sulfate precipitation and chromatography on phosphocellulose. The purified restriction endonuclease is free of exonuclease and other endonucleases.
Original language | English (US) |
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Pages (from-to) | 78-85 |
Number of pages | 8 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 76 |
Issue number | 1 |
DOIs | |
State | Published - May 9 1977 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology